Kuo K W, Chang C C
Department of Biochemistry, Kaohsiung Medical College, Taiwan, Republic of China.
Gaoxiong Yi Xue Ke Xue Za Zhi. 1990 Aug;6(8):408-17.
Modification of alpha-bungarotoxin (alpha-BuTX) with glutaraldehyde (GA), a bifunctional reagent, resulted in the formation of crosslinked derivatives. The intramolecular linked monomeric derivative as well as dimeric and higher molecular weight derivatives arising from intermolecular reactions were separated by gel filtration. The dimer and polymers were completely detoxified and the monomer lost nearly all of its lethal toxicity as well as binding activity to the nicotinic acetylcholine receptor. However, all the modified derivatives retained relatively high antigenic activity towards the antibody against native toxin. Antisera prepared by immunization with the unfractionated modified products of alpha-BuTX contained considerable amounts of a non-precipitating antibody, but much less of a precipitating antibody to alpha-BuTX, while they had the same capacity as that of anti-alpha-BuTX for neutralizing the lethal toxicity of alpha-BuTX. Furthermore, the antisera could be obtained in only half period required for anti-alpha-BuTX sera so that the detoxified GA-alpha-BuTX is an useful immunogen for the production of antibody to alpha-BuTX.
用双功能试剂戊二醛（GA）对α-银环蛇毒素（α-BuTX）进行修饰，导致形成交联衍生物。通过凝胶过滤分离分子内连接的单体衍生物以及分子间反应产生的二聚体和更高分子量的衍生物。二聚体和聚合物完全解毒，单体几乎失去了所有的致死毒性以及与烟碱型乙酰胆碱受体的结合活性。然而，所有修饰衍生物对天然毒素抗体仍保留相对较高的抗原活性。用未分级的α-BuTX修饰产物免疫制备的抗血清含有大量非沉淀抗体，但针对α-BuTX的沉淀抗体少得多，而它们中和α-BuTX致死毒性的能力与抗α-BuTX相同。此外，获得抗血清所需的时间仅为抗α-BuTX血清所需时间的一半，因此解毒的GA-α-BuTX是用于产生抗α-BuTX抗体的有用免疫原。
