[Effects of age and gender on serum levels of apolipoprotein A-I, A-II, B, C-II, C-III and E, measured by turbidimetric immunoassay, in normolipidemic Japanese elderly].

Accumulating evidence has suggested significant roles of apolipoproteins in lipoprotein metabolism. Therefore it is important to measure serum levels of apolipoproteins to understand the pathophysiology of lipid metabolism. The newly developed turbidimetric immunoassay (TIA) method for the determination of serum apolipoprotein concentration is the first method which can be employed with autoanalyzer, and is expected to be used widely. The standard values of serum concentrations of apolipoproteins measured by the TIA method for Japanese adults and children have been reported recently, but not yet for the elderly. Therefore, we investigated serum concentrations of apolipoproteins (A-I, A-II, B, C-II, C-III and E) in healthy normolipidemic elderly, aged 65-99 years, in order to obtain the standard values of apolipoproteins for the elderly. The subjects consisted of 84 males and 91 females. They had normal serum values for total cholesterol (TC) (120-220 mg/dl) and triglyceride (TG) (50-150 mg/dl). No one demonstrated clinical, biochemical or haematological evidence of cardiovascular, peripheral-vascular, cerebrovascular, hepatic, renal or endocrine disorders. Between-assay and intra-assay coefficient variations were approximately 5% and approximately 1.5%, respectively, in all apolipoproteins examined.(ABSTRACT TRUNCATED AT 250 WORDS)