Chazotte Brad
Cold Spring Harb Protoc. 2011 Jan 1;2011(1):pdb.prot5555. doi: 10.1101/pdb.prot5555.
Cell membranes can be labeled with cationic lipophilic fluorescent carbocyanine dyes that function as phospholipid analogs. These dyes, such as DiI-C(16)(3) (1,1'-dihexadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate), label the plasma membrane and (eventually) label all of the membranes within a living cell. The dyes can be also used with model membranes. Carbocyanine dyes are commercially available in different carbon chain lengths (e.g., C(12), C(14), C(18), and C(22)). Depending on the fatty acid composition of the phospholipids in the membrane studied with respect to chain length and degree of saturation, DiIs with different chain lengths can provide optimal incorporation and accurate reporting of membrane motions. DiI-labeled cells can be studied using fluorescence microscopy or fluorescence recovery after photobleaching (FRAP), as well as other techniques.
细胞膜可用作为磷脂类似物的阳离子亲脂性荧光碳菁染料进行标记。这些染料，如DiI-C(16)(3)（1,1'-二己基-3,3,3',3'-四甲基吲哚碳菁高氯酸盐），标记质膜，并（最终）标记活细胞内的所有膜。这些染料也可用于模型膜。碳菁染料有不同碳链长度（如C(12)、C(14)、C(18)和C(22)）的市售产品。根据所研究膜中磷脂的脂肪酸组成（相对于链长度和饱和度），不同链长度的DiI可提供最佳掺入并准确报告膜运动情况。可用荧光显微镜、光漂白后荧光恢复（FRAP）以及其他技术研究DiI标记的细胞。
