Medical University of Gdańsk, Division of Propedeutics of Oncology, Gdańsk, Poland.
Dis Markers. 2010;29(5):207-12. doi: 10.3233/DMA-2010-0742.
Estimation of HER2 membranous expression is routinely used in breast and gastric cancers, as both a prognostic and a predictive factor. To date there is no evidence for similar application of HER2 expression in colorectal cancer (CRC) cells. In CRC, HER2 is sometimes overexpressed in the cell membrane and very often in the cytoplasm. This study was conducted to determine possible correlations between both membranous and cytoplasmatic expression of HER2 in CRC cells and the outcome of the disease. The prognostic significance of combined staining intensity in the cell membrane and cytoplasm in the entire CRC cell was also investigated. HER2 expression in resectable colorectal adenocarcinoma cells was evaluated by immunohistochemistry in specimens taken from 202 patients. The percentage of cancer cells with membranous or cytoplasmatic reactions and the staining intensity of the reaction in the whole cell were recorded. A membranous reaction was present in 27% of cases, and cytoplasmatic reaction in 66% of cases. The total staining intensity in the entire cell was evaluated as moderate (2+) in 32% of cases and strong (3+) staining in 15%. There was no correlation found between either membranous or cytoplasmatic HER2 expression and survival. Furthermore combined staining intensity did not provide any prognostic information. We conclude that HER2 expression in CRC does not correlate with prognosis.
HER2 膜表达的评估通常用于乳腺癌和胃癌，既是预后因素也是预测因素。迄今为止，尚无证据表明 HER2 表达可类似地应用于结直肠癌（CRC）细胞。在 CRC 中，HER2 有时在细胞膜上过表达，并且常常在细胞质中过表达。本研究旨在确定 CRC 细胞中 HER2 的膜和细胞质表达之间的可能相关性及其与疾病结局的关系。还研究了整个 CRC 细胞中细胞膜和细胞质联合染色强度的预后意义。对 202 例可切除结直肠腺癌细胞的标本进行免疫组织化学评估，以评估 HER2 的表达。记录具有膜或细胞质反应的癌细胞百分比和整个细胞中反应的染色强度。在 27%的病例中存在膜反应，在 66%的病例中存在细胞质反应。整个细胞的总染色强度在 32%的病例中评为中度（2+），在 15%的病例中评为强（3+）染色。无论是膜 HER2 表达还是细胞质 HER2 表达均与生存无相关性。此外，联合染色强度未提供任何预后信息。我们得出结论，CRC 中的 HER2 表达与预后无关。
