肠炎沙门氏菌肠炎血清型菌株中类Dps蛋白SEp22十二聚体的结构
Structures of the SEp22 dodecamer, a Dps-like protein from Salmonella enterica subsp. enterica serovar Enteritidis.
作者信息
Miyamoto Takanori, Asahina Yasuko, Miyazaki Shohei, Shimizu Hidetoshi, Ohto Umeharu, Noguchi Shuji, Satow Yoshinori
机构信息
Graduate School of Pharmaceutical Sciences, The University of Tokyo, Japan.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Jan 1;67(Pt 1):17-22. doi: 10.1107/S1744309110043113. Epub 2010 Dec 21.
Abstract
The crystal structure of SEp22, a DNA-binding protein from starved cells from Salmonella enterica subsp. enterica serovar Enteritidis, has been determined in two forms: the native state at 1.25 Å resolution and an iron-soaked form at 1.30 Å resolution. The SEp22 protomers form a dodecameric shell with 23 symmetry and a single iron ion per protomer was found at the ferroxidase centre in the iron-soaked form. Along the threefold axes of the 23 symmetry, hydrophilic Asp channels that consist of Asp146 were found. Iron ions may flow into the cavity of the dodecameric shell through the Asp channels.
摘要
肠炎沙门氏菌肠炎亚种肠炎血清型饥饿细胞中的一种DNA结合蛋白SEp22的晶体结构已通过两种形式确定：分辨率为1.25 Å的天然状态和分辨率为1.30 Å的铁浸泡形式。SEp22原体形成具有23对称性的十二聚体壳，在铁浸泡形式的铁氧化酶中心发现每个原体有一个铁离子。沿着23对称性的三重轴，发现了由Asp146组成的亲水性天冬氨酸通道。铁离子可能通过天冬氨酸通道流入十二聚体壳的腔内。
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