Moraes Christopher, Sun Yu, Simmons Craig A
Department of Mechanical and Industrial Engineering, University of Toronto, Canada.
J Vis Exp. 2010 Dec 26(46):2224. doi: 10.3791/2224.
The ability to systematically probe in vitro cellular response to combinations of mechanobiological stimuli for tissue engineering, drug discovery or fundamental cell biology studies is limited by current bioreactor technologies, which cannot simultaneously apply a variety of mechanical stimuli to cultured cells. In order to address this issue, we have developed a series of microfabricated platforms designed to screen for the effects of mechanical stimuli in a high-throughput format. In this protocol, we demonstrate the fabrication of a microactuator array of vertically displaced posts on which the technology is based, and further demonstrate how this base technology can be modified to conduct high-throughput mechanically dynamic cell culture in both two-dimensional and three-dimensional culture paradigms.
当前的生物反应器技术限制了对组织工程、药物发现或基础细胞生物学研究中机械生物学刺激组合的体外细胞反应进行系统探究的能力，因为这些技术无法同时对培养的细胞施加多种机械刺激。为了解决这个问题，我们开发了一系列微制造平台，旨在以高通量形式筛选机械刺激的效果。在本方案中，我们展示了该技术所基于的垂直位移柱微致动器阵列的制造，并进一步展示了如何修改这种基础技术以在二维和三维培养模式下进行高通量机械动态细胞培养。
