Department of Chemistry and Bioengineering, Faculty of Fundamental Sciences, Vilnius Gediminas Technical University, Sauletekio al. 11, LT-10223, Vilnius-40, Lithuania.
Mol Biotechnol. 2011 Sep;49(1):11-8. doi: 10.1007/s12033-010-9370-6.
The influence of L-arginine on the temperature-induced aggregation of porcine and mink growth hormones was studied by fluorescence spectroscopy. It was found that L-arginine suppresses the heat-induced aggregation. Moreover, the analysis of L-arginine interaction with the native proteins by fluorescence spectroscopy and circular dichroism spectroscopy revealed no significant changes in their native structure. On the basis of the results, L-arginine could be considered as a potential additive for the prevention of storage and temperature-related denaturation and aggregation of veterinary growth hormones.
荧光光谱法研究了 L-精氨酸对猪和水貂生长激素在温度诱导下聚集的影响。结果表明，L-精氨酸能抑制热诱导聚集。此外，通过荧光光谱法和圆二色性光谱法分析 L-精氨酸与天然蛋白质的相互作用，发现其天然结构没有明显变化。基于这些结果，L-精氨酸可以被认为是一种潜在的添加剂，用于预防兽用生长激素在储存和温度相关变性和聚集。
