Kombu Rajan S, Brunengraber Henri, Puchowicz Michelle A
Department of Nutrition, Mouse Metabolic Phenotyping Center, Case Western Reserve University, Cleveland, OH, USA.
Methods Mol Biol. 2011;708:147-57. doi: 10.1007/978-1-61737-985-7_8.
Researchers view analysis of the citric acid cycle (CAC) intermediates as a metabolomic approach to identifying unexpected correlations between apparently related and unrelated pathways of metabolism. Relationships of the CAC intermediates, as measured by their concentrations and relative ratios, offer useful information to understanding interrelationships between the CAC and metabolic pathways under various physiological and pathological conditions. This chapter presents a relatively simple method that is sensitive for simultaneously measuring concentrations of CAC intermediates (relative and absolute) and other related intermediates of energy metabolism using gas chromatography-mass spectrometry.
研究人员将柠檬酸循环（CAC）中间体的分析视为一种代谢组学方法，用于识别明显相关和不相关代谢途径之间意想不到的关联。通过其浓度和相对比率衡量的CAC中间体之间的关系，为理解各种生理和病理条件下CAC与代谢途径之间的相互关系提供了有用信息。本章介绍了一种相对简单的方法，该方法通过气相色谱 - 质谱联用技术，能够灵敏地同时测量CAC中间体（相对和绝对）以及能量代谢的其他相关中间体的浓度。
