Department of Biophysics, Panjab University, Chandigarh, India.
Nutr Cancer. 2011;63(2):212-7. doi: 10.1080/01635581.2011.523167.
The present study was conducted to evaluate the kinetics of zinc utilization during the formation of colon carcinoma in an animal model of colon carcinogenesis. The rats were segregated into 4 groups: untreated control, 1,2-dimethylhydrazine (DMH) treated, zinc treated, and DMH+zinc treated. Colon carcinogenesis was initiated through weekly subcutaneous injections of DMH (30 mg/kg body weight) for 8 wk. Zinc (in the form of zinc sulphate) was supplemented at a dose level of 227 mg/L in drinking water, ad libitum for the entire duration of study. Whole body (65)Zn kinetics followed two-compartment kinetics, with Tb(1) representing the initial fast component of the biological half-life and Tb(2), the slower component. The Tb(1) component showed a significant elevation while the Tb(2) component was significantly diminished in DMH-treated rats, which, however, got normalized following zinc supplementation. The biodistribution and subcellular distribution of (65)Zn was significantly affected in DMH-treated rats when compared to normal control rats. However, zinc significantly reversed the altered (65)Zn uptake in different organs and various fractions of colon. The present study for the first time demonstrated a faster mobilization of zinc during initiation of experimentally induced colon carcinoma and provides a physiological basis for the role of zinc in colon tumorigenesis.
本研究旨在评估动物结直肠癌发生模型中锌利用的动力学变化。将大鼠分为 4 组：未处理对照组、1,2-二甲基肼（DMH）处理组、锌处理组和 DMH+锌处理组。通过每周皮下注射 DMH（30mg/kg 体重）8 周来启动结直肠癌的发生。锌（以硫酸锌的形式）以 227mg/L 的剂量水平添加到饮用水中，自由摄取整个研究期间。全身（65）Zn 动力学遵循双室动力学，Tb(1)表示生物半衰期的初始快速成分，Tb(2)表示较慢的成分。在 DMH 处理的大鼠中，Tb(1)成分显著升高，而 Tb(2)成分显著降低，但补充锌后得到了正常化。与正常对照组大鼠相比，DMH 处理的大鼠的（65）Zn 分布和亚细胞分布受到显著影响。然而，锌显著逆转了不同器官和结肠不同部位中改变的（65）Zn 摄取。本研究首次证明了在实验诱导的结肠癌发生过程中锌的更快动员，并为锌在结肠肿瘤发生中的作用提供了生理基础。
