[BRL条件培养基在胚胎干细胞培养中的应用]
[Application of BRL conditioned medium in ES cells culture].
作者信息
Zeng Q T, Zhu W Q, Cao L S, Qin R Z, Zhang G Q, Jiang J Q
机构信息
Institute of Cardiovascular Diseases, Tongji Medical College, Huazhong Science and Technology University, Wuhan 430022, China.
出版信息
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2001 Nov;17(4):398-401.
AIM
To study the method of using BRL conditioned medium in ES cells culture.
METHODS
ES cells were cultured in BRL conditioned medium, MEF feeder layer, and BRL conditioned medium plus MEF feeder layer respectively. Both the numbers of ES cells and embryonic bodies and the differentiated cells around the ES cell colonies at certain stages were studied to find the differences of growing speed of ES cells in three culture mediums.
RESULTS
On promoting the growth and maintaining the undifferentiated state of ES cells at the early stage after recovery, the effects of BRL conditioned medium were less than those of MEF feeder layer and those of BRL conditioned medium plus MEF feeder layer (P < 0.01), and there was no difference between MEF feeder layer and BRL conditioned medium plus MEF feeder layer (P > 0.05).
CONCLUSION
ES cells should be cultivated on MEF feeder layer or in BRL conditioned medium plus MEF feeder layer at the early stage after recovering.
目的
研究在胚胎干细胞（ES细胞）培养中使用大鼠肝细胞（BRL）条件培养基的方法。
方法
ES细胞分别在BRL条件培养基、小鼠胚胎成纤维细胞（MEF）饲养层以及BRL条件培养基加MEF饲养层中培养。研究ES细胞数量、胚体数量以及特定阶段ES细胞集落周围的分化细胞，以找出三种培养基中ES细胞生长速度的差异。
结果
在恢复后的早期，关于促进ES细胞生长和维持其未分化状态，BRL条件培养基的效果小于MEF饲养层和BRL条件培养基加MEF饲养层的效果（P<0.01），且MEF饲养层和BRL条件培养基加MEF饲养层之间无差异（P>0.05）。
结论
恢复后的早期，ES细胞应在MEF饲养层上或在BRL条件培养基加MEF饲养层中培养。
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