[肾上腺髓质素在哮喘豚鼠肺组织中的表达变化及其对离体气管条收缩的影响]
[Change of the expression of adrenomedullin in lung and effect on contraction of isolated tracheal strip of asthmatic guinea pigs].
作者信息
Wu Xiu-Ming, Dai Ai-Guo, Li Ji-Hong, Zhou Shi-Yi
机构信息
Department of Respiratory Medicine, Third Affiliated Hospitel, Nanhua University, Hengyang 421900, China.
出版信息
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2003 Feb;19(1):70-3.
AIM
To study the formation and localization of ADM mRNA in lung tissues and investigate the effects of ADM on isolated tracheal strip contraction induced by histamine in asthmatic guinea pig.
METHODS
The guinea pigs (n = 22) were randomly divided into two groups of 11 each: asthmatic group and control group. The formation and localization of ADM mRMA were observed by in site hybridization. The effect of exogenous ADM on contractions of isolated tracheal strip of the asthmatic guinea pigs to histamine was examined.
RESULTS
There were strong positive expression for ADM mRNA in airway epithelial cells (AEC), smooth muscle cells (ASMC) in asthmatic group. The control group showed significantly decreased number of ADM mRNA positive cells in lung tissues. From 10(-11) mol/L to 10(-7) mol/L, ADM may cause concentration depend pentiation of the isolated tracheal strip contraction induced by histamine of asthmatic group which was higher significantly compared the control group (P < 0.05). 10(-8) mol/L ADM reached the maximal relaxation, with the increasing of ADM, neither asthmatic nor control group can increase the relaxation.
CONCLUSION
There is ADM mRNA overproduction in AEC and ASMC and exogenous ADM may inhibit isolated tracheal strip contraction induced by histamine of asthmatic guinea pig, which may contribute to airway inflammation and hyperresponsiveness in asthma.
目的
研究哮喘豚鼠肺组织中肾上腺髓质素（ADM）mRNA的形成与定位，并探讨ADM对组胺诱导的离体气管条收缩的影响。
方法
将22只豚鼠随机分为两组，每组11只：哮喘组和对照组。采用原位杂交法观察ADM mRNA的形成与定位。检测外源性ADM对哮喘豚鼠离体气管条对组胺收缩反应的影响。
结果
哮喘组气道上皮细胞（AEC）、平滑肌细胞（ASMC）中ADM mRNA呈强阳性表达。对照组肺组织中ADM mRNA阳性细胞数量明显减少。从10^(-11)mol/L到10^(-7)mol/L，ADM可使哮喘组组胺诱导的离体气管条收缩呈浓度依赖性减弱，与对照组相比差异有统计学意义（P<0.05）。10^(-8)mol/L ADM时达到最大舒张，随着ADM浓度增加，哮喘组和对照组的舒张均不再增加。
结论
AEC和ASMC中存在ADM mRNA过度产生，外源性ADM可抑制哮喘豚鼠组胺诱导的离体气管条收缩，这可能与哮喘气道炎症和高反应性有关。
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