Ruuge E K, Tikhonov A N
Biofizika. 1978 Sep-Oct;23(5):839-44.
The kinetics of P700 redox-transients in leaves and isolated been and spinach chloroplasts was studied by the ESR method. P700 oxidation after a short flash (less than 10 microseconds) of white light was not resolved. P700 oxidation may be resolved only after "long" (more than 500 microseconds) flash of red light (700 or 720 nm) with a slow reduction of P700+ in the dark. In case of 500 microseconds flash exciting both photosystems (600 nm) P700 response was not observed. Fast P700+ reduction was observed if the short pulse of white light was given simultaneously with a continuous background 707 nm light. We can conclude from these data that P700+ accepts an electron from its primary donor within 10--500 microseconds.
采用电子顺磁共振(ESR)方法研究了叶片、离体蚕豆和菠菜叶绿体中P700氧化还原瞬变的动力学。短时间(小于10微秒)白光闪光后P700的氧化无法分辨。只有在“长”(超过500微秒)的红光(700或720纳米)闪光后,且在黑暗中P700+缓慢还原的情况下,P700的氧化才可以分辨。在500微秒闪光激发两个光系统(600纳米)的情况下,未观察到P700的响应。如果在连续的707纳米背景光的同时给予短脉冲白光,则观察到P700+的快速还原。从这些数据我们可以得出结论,P700+在10 - 500微秒内从其初级供体接受一个电子。