Dept. Plant Pathology and Microbiology, University of California, Riverside, CA 92521, United States.
Virus Res. 2011 Mar;156(1-2):64-71. doi: 10.1016/j.virusres.2010.12.019. Epub 2011 Jan 4.
Viruses in the genus Crinivirus infect diverse plant species and are transmitted by specific whitefly vectors, but the basis for vector specific transmission remains poorly understood. Here, we demonstrated that purified virion preparations of Lettuce chlorosis virus (LCV) contained filamentous particles that were consistently transmitted to plants by whiteflies (Bemisia tabaci biotypes A and B) following membrane feeding, suggesting that the preparations contained biologically active virions with all the components essential for specific vector transmission. We also demonstrated in sequential membrane feeding experiments that B. tabaci biotype A pre-fed with high concentrations of Lettuce infectious yellows virus (LIYV) virions followed by decreasing concentrations of LCV virions either abolished or interfered with the transmission of the latter. However, in the reverse treatment, an abolishment/interference in transmission of LIYV was not observed. These results suggest that both viruses share a common transmission pathway in B. tabaci biotype A, and factors other than virion quality and quantity may additionally influence their transmission. To begin investigating the viral determinants that are involved in mediating the whitefly transmission of LCV, virions were analyzed by Western immunoblotting. Our results showed that virions were positively identified by antisera produced against three E. coli expressed recombinant LCV capsid proteins--the major coat protein [CP], minor CP [CPm], and P60.
类莨菪病毒属的病毒感染多种植物物种，并通过特定的粉虱媒介传播，但媒介特异性传播的基础仍知之甚少。在这里，我们证明了莴苣褪绿病毒（LCV）的纯化病毒粒子制剂含有丝状颗粒，这些颗粒在通过膜喂养后被粉虱（烟粉虱生物型 A 和 B）持续传播到植物中，这表明制剂中含有具有所有成分的生物活性病毒粒子对于特定媒介传播至关重要。我们还在连续的膜喂养实验中证明，在用高浓度莴苣传染性黄化病毒（LIYV）病毒粒子预喂养烟粉虱生物型 A 后，再用逐渐降低浓度的 LCV 病毒粒子进行喂养，要么会消除要么会干扰后者的传播。然而，在相反的处理中，LIYV 的传播不会被消除/干扰。这些结果表明，这两种病毒在烟粉虱生物型 A 中共享一个共同的传播途径，除了病毒粒子的质量和数量之外，其他因素可能也会影响它们的传播。为了开始研究参与介导 LCV 粉虱传播的病毒决定因素，我们通过 Western 免疫印迹分析了病毒粒子。我们的结果表明，病毒粒子被针对三种在大肠杆菌中表达的重组 LCV 衣壳蛋白（主要衣壳蛋白 [CP]、次要 CP [CPm] 和 P60）产生的抗血清阳性识别。
