Department of Microbiology and Plant Pathology, University of California, Riverside, CA 92521, USA.
Citrus Research Institute, Southwest University, Beibei, Chongqing 400712, China.
Viruses. 2021 Aug 10;13(8):1578. doi: 10.3390/v13081578.
Lettuce infectious yellows virus is the first crinivirus for which the retention of purified virions ingested into the whitefly ( New World (NW)) vector's foregut, has been demonstrated to be a requisite for successful virus transmission. This key finding supports the hypothesis that the determinant of foregut retention and transmission is present on the virion itself. However, whether this is also true for other criniviruses has not been established. Here, we provide evidence that lettuce chlorosis virus (LCV) acquired from plants is retained in the foreguts of both the NW and Middle East-Asia Minor 1 (MEAM1) vector species and transmitted upon inoculation feeding. An association between foregut retention and transmission by NW vectors is also observed following the acquisition and inoculation feeding of LCV virions purified using a standard procedure involving 2% or 4% (/) Triton™ X-100 (TX-100). However, while virions purified with 2% or 4% TX-100 are also retained in the foreguts of MEAM1 vectors, transmission is observed with the 4% TX-100-purified virions or when more vectors are used for acquisition and inoculation feeding. These results suggest that an intrinsic difference exists between NW and MEAM1 vectors in their interactions with, and transmission of, LCV virions.
莴苣黄化病毒是首个被证明其纯化病毒粒子在被烟粉虱（新世界（NW））取食进入前肠后保留是成功传播病毒所必需的克鲁因病毒。这一关键发现支持了这样一种假设，即前肠保留和传播的决定因素存在于病毒粒子本身。然而，这是否也适用于其他克鲁因病毒还没有确定。在这里，我们提供的证据表明，从植物中获得的莴苣褪绿病毒（LCV）在前肠中被 NW 和中东-亚洲小种 1（MEAM1）两种载体物种保留，并在接种取食时传播。在 NW 载体通过获得和接种纯化的 LCV 病毒粒子进行取食后，也观察到了与前肠保留和传播之间的关联，这些病毒粒子是使用标准程序（涉及 2%或 4%（/）Triton™ X-100（TX-100））纯化的。然而，虽然用 2%或 4% TX-100 纯化的病毒粒子也在前肠中被 MEAM1 载体保留，但在用 4% TX-100 纯化的病毒粒子或使用更多的载体进行获得和接种取食时，会观察到传播。这些结果表明，NW 和 MEAM1 载体在与 LCV 病毒粒子相互作用和传播方面存在内在差异。
