Department of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University, 4700 Hillsborough Street, Raleigh, NC 27606, USA. Isabel
Vaccine. 2011 Feb 11;29(8):1583-94. doi: 10.1016/j.vaccine.2010.12.079. Epub 2011 Jan 5.
Transcription of cytokine genes was evaluated in the lung and spleen of chickens vaccinated with various serotype 1 Marek's disease (MD) vaccines. Three vaccine pairs/series, each consisting of one or two high protective (HP) and one low protective (LP) vaccine, were used. Vaccinated chickens had increased transcripts of IFN-γ in the lung and spleen at 3, 5, and 10 days post vaccination (dpv) compared to unvaccinated control chickens. In addition, transient increase of cytokine transcripts (iNOS, IL-1β, IL-18, IL-8, and IL-6 in the lung and iNOS, IL-18, and IL-6 in the spleen) was detected. Compared to chickens vaccinated with LP vaccines, HP vaccinated chickens had increased transcripts of iNOS at 5 dpv but decreased transcripts of IL-6, IL-8, and IL-18 at 10 dpv in the lung. HP vaccinated chickens had increased IFN-γ in the spleen at 3 and at 10 dpv. This study demonstrated that MD vaccines administered subcutaneously elicit a pulmonary immune response and identified differences in the cytokine gene expression between HP and LP vaccinated chickens.
本研究评估了不同血清型 1 型马立克氏病（MD）疫苗接种鸡的肺部和脾脏中细胞因子基因的转录情况。使用了三对/系列疫苗，每对/系列疫苗由一个或两个高保护（HP）和一个低保护（LP）疫苗组成。与未接种对照鸡相比，接种鸡在接种后 3、5 和 10 天（dpv）肺部和脾脏中 IFN-γ 的转录本增加。此外，还检测到细胞因子转录本（肺部的 iNOS、IL-1β、IL-18、IL-8 和 IL-6 以及脾脏中的 iNOS、IL-18 和 IL-6）的短暂增加。与接种 LP 疫苗的鸡相比，接种 HP 疫苗的鸡在 5 dpv 时肺部的 iNOS 转录本增加，但在 10 dpv 时 IL-6、IL-8 和 IL-18 的转录本减少。接种 HP 疫苗的鸡在 3 和 10 dpv 时脾脏中 IFN-γ 的转录本增加。本研究表明，皮下给予 MD 疫苗可引起肺部免疫反应，并鉴定出 HP 和 LP 接种鸡之间细胞因子基因表达的差异。
