Department of Chemistry, Georgia State University, Atlanta, GA 30302-4098, USA.
Bioorg Med Chem Lett. 2011 Feb 1;21(3):1047-51. doi: 10.1016/j.bmcl.2010.12.009. Epub 2010 Dec 8.
We report the synthesis of photonuclease 3 consisting of two acridine rings joined by a 2,6-bis(aminomethyl)pyridine copper-binding linker. In reactions containing micromolar concentrations of 3, irradiation at 419 nm produces efficient, copper(II)-dependent cleavage of plasmid DNA in the presence of the high concentrations of salt that exist in the cell nucleus (150 mM NaCl and 260 mM KCl). The DNA interactions of 3 are compared to an analogous bis-acridine (4) containing a more flexible 2,6-bis{[(methoxycarbonylamino)-ethyl]methylaminomethyl}pyridine unit.
我们报告了由两个吖啶环通过 2,6-双（氨甲基）吡啶铜结合连接体连接而成的光解酶 3 的合成。在含有微摩尔浓度 3 的反应中，在存在细胞核中存在的高浓度盐（150 mM NaCl 和 260 mM KCl）的情况下，在 419nm 照射下产生有效的、铜(II)依赖性的质粒 DNA 切割。3 的 DNA 相互作用与含有更灵活的 2,6-双{[(甲氧基羰基氨基)-乙基]甲基氨甲基}吡啶单元的类似双吖啶（4）进行了比较。
