Discipline of Marine Biotechnology and Ecology, Central Salt and Marine Chemicals Research Institute (Council of Scientific and Industrial Research), G. B. Marg, Bhavnagar - 364 021, Gujarat, India.
Helmholtz Zentrum München, German Research Center for Environmental Health (GmbH), Department Microbe-Plant Interactions, Ingolstaedter Landstrasse 1, D-85764 Neuherberg, Germany.
Int J Syst Evol Microbiol. 2011 Dec;61(Pt 12):2799-2804. doi: 10.1099/ijs.0.023176-0. Epub 2011 Jan 7.
A Gram-positive-staining, aerobic, non-motile, coccoid shaped, halotolerant bacterium (strain JG 06(T)) was isolated from the roots of Salicornia brachiata, an extreme halophyte. Phylogenetic analysis based on 16S rRNA gene sequence showed that the novel strain had sequence similarities of 99.2% to Brachybacterium paraconglomeratum JCM 11608(T), 99.0% to Brachybacterium conglomeratum DSM 10241 and 98.2% to Brachybacterium faecium DSM 4810(T). DNA-DNA hybridization with B. paraconglomeratum DSM 46341(T), B. conglomeratum DSM 10241(T), B. faecium DSM 4810(T), Brachybacterium tyrofermentans DSM 10673(T), Brachybacterium alimentarium DSM 10672(T), Brachybacterium fresconsis DSM 14564(T), Brachybacterium sacelli DSM 14566(T) and Brachybacterium muris DSM 15460(T) resulted in reassociation values of 36.2%, 36.5%, 35.8%, 27.6%, 27.9%, 28.2%, 28.7% and 11.2%, respectively. The peptidoglycan type of strain JG 06(T) was variant A4γ. The menaquinone content was MK7 (100%). The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, monogalactosyl diglyceride, three unidentified phospholipids and three glycolipids. The predominant fatty acid was anteiso-C(15:0) (52.07%); significant amounts of iso-C(16:0)(12.38%), iso-C(15:0 )(8.59%) and anteiso-C(17:0)(10.03%) were also present. The G+C content of the DNA was 73.0 mol%. The strain formed a growth pellicle in nitrogen-free semisolid NFb medium containing NaCl at levels of up to 4% (w/v) and reduced acetylene to ethylene, a result indicative of N(2) fixation. In nutrient broth medium the novel strain grew at NaCl concentrations up to 15% (w/v). It also had the ability to produce indole-3-acetic acid (IAA) and siderophores, utilized 1-aminocyclopropane-1-carboxylate (ACC) as a sole source of nitrogen and possessed the ACC deaminase enzyme. On the basis of physiological, biochemical data and phylogenetic analyses, strain JG 06(T) should be placed in the genus Brachybacterium. Strain JG 06(T) represents a novel species of the genus Brachybacterium for which the name Brachybacterium saurashtrense sp. nov. is proposed (type strain JG 06(T)=DSM 23186(T)=IMCC 252(T)).
一株革兰氏阳性、需氧、不运动、球形、耐盐的细菌（菌株 JG06(T)）从盐角草 Salicornia brachiata 的根部分离得到，盐角草是一种极端盐生植物。基于 16S rRNA 基因序列的系统发育分析表明，新菌株与 Brachybacterium paraconglomeratum JCM 11608(T)、Brachybacterium conglomeratum DSM 10241 和 Brachybacterium faecium DSM 4810(T)的序列相似性分别为 99.2%、99.0%和 98.2%。与 Brachybacterium paraconglomeratum DSM 46341(T)、Brachybacterium conglomeratum DSM 10241(T)、Brachybacterium faecium DSM 4810(T)、Brachybacterium tyrofermentans DSM 10673(T)、Brachybacterium alimentarium DSM 10672(T)、Brachybacterium fresconsis DSM 14564(T)、Brachybacterium sacelli DSM 14566(T)和 Brachybacterium muris DSM 15460(T)的 DNA-DNA 杂交产生的再结合值分别为 36.2%、36.5%、35.8%、27.6%、27.9%、28.2%、28.7%和 11.2%。菌株 JG06(T)的肽聚糖类型为变体 A4γ。其类异戊二烯醌为 MK7（100%）。极性脂谱由双磷脂酰甘油、磷脂酰甘油、单半乳糖基二甘油酯、三种未鉴定的磷脂和三种糖脂组成。优势脂肪酸为 anteiso-C(15:0)（52.07%）；还存在大量的 iso-C(16:0)(12.38%)、iso-C(15:0)(8.59%)和 anteiso-C(17:0)(10.03%)。DNA 的 G+C 含量为 73.0mol%。该菌株在含高达 4%（w/v）NaCl 的无氮半固体 NFb 培养基中形成生长膜，并将乙炔还原为乙烯，这表明具有固氮能力。在营养肉汤培养基中，新菌株可在高达 15%（w/v）的 NaCl 浓度下生长。它还具有产生吲哚-3-乙酸（IAA）和铁载体的能力，利用 1-氨基环丙烷-1-羧酸（ACC）作为唯一氮源，并具有 ACC 脱氨酶酶。基于生理生化数据和系统发育分析，菌株 JG06(T)应归入短杆菌属。菌株 JG06(T)代表短杆菌属的一个新种，建议将其命名为 Saurashtra 短杆菌（Brachybacterium saurashtrense sp. nov.）。（模式菌株 JG06(T)=DSM 23186(T)=IMCC 252(T)）。
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