Life Sciences Institute, Zunyi Medical University, Zunyi, 563006, People's Republic of China.
Key Laboratory of Major Diseases in Children, Ministry of Education, National Key Discipline of Pediatrics (Capital Medical University), Beijing Key Laboratory of Pediatric Respiratory Infection Disease, National Clinical Research Center for Respiratory Diseases, National Center for Children's Health, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, Beijing, 100045, People's Republic of China.
Antonie Van Leeuwenhoek. 2021 Jun;114(6):875-884. doi: 10.1007/s10482-021-01565-z. Epub 2021 Apr 1.
A novel, aerobic, moderately halophilic Gram-positive actinomycete, strain MASK1Z-5 was isolated from a surface-sterilized branch of Bruguiera gymnoirhiza in Shankou Mangrove Nature Reserve, Guangxi, China. The taxonomic position of the strain was investigated using a polyphasic approach. Strain MASK1Z-5 tolerated up to 20% (w/v) NaCl (optimum 0-7%), and grew at pH 5.0-12.0 (optimum pH 7.0-8.0), 20-37 °C (optimum 30 °C). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain MASK1Z-5 belonged to the genus Brachybacterium, and showed the highest 16S rRNA gene sequence similarity of 98.0% to B. endophyticum M1HQ-2. The cell-wall peptidoglycan contained meso-diaminopimelic acid as diagnostic diamino acid. MK-7 was the predominant menaquinone. The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, one unidentified glycolipid and three unidentified lipids. The major fatty acids were anteiso-C, iso-C and anteiso-C. The DNA G + C content was calculated to be 71.8 mol% based on the whole genome sequence. The estimated values of average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) based on whole genome sequences between strain MASK1Z-5 and B. endophyticum M1HQ-2 were 81.8% and 25.0%, respectively. The phenotypic, chemotaxonomic and genotypic properties clearly indicated that strain MASK1Z-5 represents a novel species within the genus Brachybacterium, for which the name Brachybacterium halotolerans sp. nov. is proposed. The type strain is MASK1Z-5 (= CGMCC1.18660 = JCM 34339).
一株新的、好氧、中度嗜盐的革兰氏阳性放线菌，菌株 MASK1Z-5 是从中国广西山口红树林自然保护区消毒的白骨壤树枝上分离出来的。该菌株的分类地位采用多相研究方法进行了研究。菌株 MASK1Z-5 可耐受高达 20%（w/v）的 NaCl（最佳 0-7%），并在 pH 5.0-12.0（最佳 pH 7.0-8.0）、20-37°C（最佳 30°C）下生长。基于 16S rRNA 基因序列的系统发育分析表明，菌株 MASK1Z-5 属于短杆菌属，与 B. endophyticum M1HQ-2 的 16S rRNA 基因序列相似度最高，为 98.0%。细胞壁肽聚糖含有meso-二氨基庚二酸作为诊断二氨基酸。MK-7 是主要的menaquinone。极性脂包括双磷脂酰甘油、磷脂酰甘油、一种未鉴定的糖脂和三种未鉴定的脂类。主要脂肪酸为anteiso-C、iso-C 和 anteiso-C。根据全基因组序列，DNA G+C 含量计算为 71.8 mol%。根据全基因组序列，菌株 MASK1Z-5 和 B. endophyticum M1HQ-2 之间的平均核苷酸同一性（ANI）和数字 DNA-DNA 杂交（dDDH）的估计值分别为 81.8%和 25.0%。表型、化学分类和基因型特性清楚地表明，菌株 MASK1Z-5 代表短杆菌属中的一个新种，因此建议将其命名为耐盐短杆菌新种。模式菌株为 MASK1Z-5（=CGMCC 1.18660=JCM 34339）。
