Flores M, Ford E G, Janda J M
Microbial Diseases Laboratory, California Department of Health Services, Berkeley 94704.
J Clin Microbiol. 1990 Sep;28(9):2142-4. doi: 10.1128/jcm.28.9.2142-2144.1990.
A comparative study to determine beta-D-galactosidase activity among 171 strains of aerobic actinomycetes (including mycobacteria and rhodococci) was performed by using two growth media and four O-nitrophenyl-beta-D-galactopyranoside (ONPG) substrates. The ONPG test was found to be a valuable screening test to differentiate between the ONPG-positive Nocardia spp. and the rapidly growing ONPG-negative mycobacteria and rhodococci. However, ONPG results varied significantly depending on the growth medium and test substrate used.
通过使用两种生长培养基和四种邻硝基苯-β-D-吡喃半乳糖苷(ONPG)底物,对171株需氧放线菌(包括分枝杆菌和红球菌)的β-D-半乳糖苷酶活性进行了比较研究。发现ONPG试验是区分ONPG阳性诺卡氏菌属与快速生长的ONPG阴性分枝杆菌和红球菌的有价值的筛选试验。然而,ONPG结果根据所用的生长培养基和试验底物而有显著差异。