利用铁载体检测培养基、乙二醇降解及β-半乳糖苷酶活性对诺卡氏菌、红球菌、链霉菌和快速生长的分枝杆菌属进行早期初步鉴别
Use of a siderophore detection medium, ethylene glycol degradation, and beta-galactosidase activity in the early presumptive differentiation of Nocardia, Rhodococcus, Streptomyces, and rapidly growing Mycobacterium species.
作者信息
Fiss E, Brooks G F
机构信息
Department of Laboratory, University of California, San Francisco 94143.
出版信息
J Clin Microbiol. 1991 Jul;29(7):1533-5. doi: 10.1128/jcm.29.7.1533-1535.1991.
Abstract
A simplified scheme for the presumptive early identification of Nocardia, Rhodococcus, rapidly growing Mycobacterium, and Streptomyces species is presented. The Nocardia and Streptomyces spp. and the Mycobacterium spp. were positive. The spp. were positive for siderophore activity, but only 25% of the Rhodococcus spp. were positive. The Rhodococcus and Mycobacterium spp. were negative for beta-galactosidase, while the Nocardia and Streptomyces spp. were positive. The Nocardia and Streptomyces spp. and the Mycobacterium spp. were negative for ethylene glycol degradation, while 75% of the Rhodococcus spp. were positive. In combination, these tests were useful for differentiating Mycobacterium, Rhodococcus, and Nocardia species but did not differentiate Nocardia from Streptomyces species.
摘要
本文提出了一种用于诺卡氏菌、红球菌、快速生长分枝杆菌和链霉菌属菌种早期初步鉴定的简化方案。诺卡氏菌和链霉菌属以及分枝杆菌属呈阳性。这些菌种的铁载体活性呈阳性，但只有25%的红球菌属呈阳性。红球菌属和分枝杆菌属的β-半乳糖苷酶呈阴性，而诺卡氏菌和链霉菌属呈阳性。诺卡氏菌和链霉菌属以及分枝杆菌属的乙二醇降解呈阴性，而75%的红球菌属呈阳性。综合来看，这些试验有助于区分分枝杆菌属、红球菌属和诺卡氏菌属菌种，但无法区分诺卡氏菌属和链霉菌属菌种。
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