在活细胞中对蛋白质胆固醇酯化进行生物正交化学标记。
Bioorthogonal chemical tagging of protein cholesterylation in living cells.
机构信息
Department of Chemistry, Imperial College London, Exhibition Rd., London SW7 2AZ, UK.
出版信息
Chem Commun (Camb). 2011 Apr 14;47(14):4081-3. doi: 10.1039/c0cc04710d. Epub 2011 Jan 11.
PMID:21221452
Abstract
We report the first chemical probe for bioorthogonal chemical tagging of post-translationally cholesterylated proteins with an azide in living cells. This enables rapid multiplexed fluorescence detection and affinity labelling of protein cholesterylation, as exemplified by Sonic hedgehog protein, opening up new approaches for the de novo identification of cholesterylated proteins.
摘要
我们报道了第一个用于在活细胞中对胆固醇化的翻译后蛋白质进行生物正交化学标记的化学探针，该探针带有叠氮化物。这使得蛋白质胆固醇化的快速多重荧光检测和亲和标记成为可能，例如 Sonic hedgehog 蛋白，为新的胆固醇化蛋白质的从头鉴定开辟了新的途径。
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