Faculty of Animal Bioengineering, University of Warmia and Mazury, Olsztyn, Poland.
Biochem Genet. 2011 Jun;49(5-6):303-12. doi: 10.1007/s10528-010-9408-2. Epub 2011 Jan 11.
The allele, genotype, and haplotype frequencies among 837 Polish Holstein-Friesian cattle were determined at two regulatory indel polymorphisms of the PRNP gene. Allele frequencies at the 23 bp indel promoter polymorphism were 0.622 (del) and 0.378 (ins), with 0.613 and 0.387 in sires and 0.633 and 0.366 in dams. Allele frequencies at the 12 bp indel intron polymorphism were 0.527 (del) and 0.473 (ins), with 0.529 and 0.471 in sires and 0.543 and 0.456 in dams. Four haplotypes were identified in this population (23-12del, 23-12ins, 23del-12ins, and 23ins-12del). Haplotype 23-12del occurred most frequently in both sire and dam groups. Comparative analysis of Polish Holstein-Friesian and German Holstein populations revealed a similar genetic structure for the 23 bp indel polymorphism and a significantly different one for the 12 bp indel polymorphism. In allele and haplotype analysis, significant differences were observed between the Polish Holstein-Friesian population and a BSE-free German Holstein population.
在两个 PRNP 基因调控插入缺失多态性位点上，测定了 837 头波兰荷斯坦-弗里生牛的等位基因、基因型和单倍型频率。23bp 插入缺失启动子多态性的等位基因频率为 0.622（del）和 0.378（ins），在公牛中为 0.613 和 0.387，在母牛中为 0.633 和 0.366。12bp 插入缺失内含子多态性的等位基因频率为 0.527（del）和 0.473（ins），在公牛中为 0.529 和 0.471，在母牛中为 0.543 和 0.456。在该群体中鉴定出了 4 种单倍型（23-12del、23-12ins、23del-12ins 和 23ins-12del）。23-12del 单倍型在公牛和母牛组中均最为常见。波兰荷斯坦-弗里生牛和德国荷斯坦牛群体的比较分析表明，23bp 插入缺失多态性具有相似的遗传结构，而 12bp 插入缺失多态性则存在显著差异。在等位基因和单倍型分析中，波兰荷斯坦-弗里生牛群体与无 BSE 的德国荷斯坦牛群体存在显著差异。
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