ISG15靶蛋白的鉴定与验证
Identification and Validation of ISG15 Target Proteins.
作者信息
Durfee Larissa A, Huibregtse Jon M
机构信息
Section of Molecular Genetics and Microbiology, Institute for Cellular and Molecular Biology, The University of Texas at Austin, Austin, Texas, 78/12, USA.
出版信息
Subcell Biochem. 2010;54:228-37. doi: 10.1007/978-1-4419-6676-6_18.
Abstract
ISG15 is an interferon-induced ubiquitin-like protein (Ubl) that has antiviral properties. The core E1, E2 and E3 enzymes for conjugation of human ISG15 are Ube1L, UbcH8 and Herc5, all of which are induced at the transcriptional level by Type 1 interferon signaling. Several proteomics studies have, together, identified over 300 cellular proteins as ISG15 targets. These targets include a broad range of constitutively expressed proteins and approximately 15 interferon-induced proteins. This chapter provides an overview of the target identification process and the validation of these targets. We also discuss the limited number of examples where the biochemical effect of ISG15 conjugation on target proteins has been characterized.
摘要
ISG15是一种具有抗病毒特性的干扰素诱导的类泛素蛋白（Ubl）。人ISG15缀合的核心E1、E2和E3酶分别是Ube1L、UbcH8和Herc5，它们均在转录水平上由I型干扰素信号诱导产生。多项蛋白质组学研究共同鉴定出超过300种细胞蛋白为ISG15的靶标。这些靶标包括多种组成性表达的蛋白以及约15种干扰素诱导蛋白。本章概述了靶标鉴定过程以及对这些靶标的验证。我们还讨论了为数不多的已对ISG15缀合对靶蛋白的生化效应进行表征的实例。
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ISG15 Arg151 and the ISG15-conjugating enzyme UbE1L are important for innate immune control of Sindbis virus.ISG15的第151位精氨酸以及ISG15缀合酶UbE1L对辛德毕斯病毒的天然免疫控制很重要。
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