Avram and Stella Goldstein-Goren Department of Biotechnology Engineering, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel.
Langmuir. 2011 Mar 1;27(5):1547-50. doi: 10.1021/la1041234. Epub 2011 Jan 11.
We describe the fabrication of polymer nanofibers with entrapped molecularly imprinted polymer (MIP) nanoparticles and study their possible use in a fluorescence-based biosensor application. The MIP was imprinted with the fluorescent amino acid derivative dansyl-L-phenylalanine. Poly(vinyl alcohol) was used as a support for MIP nanoparticles because it is water-soluble and can be spun into very thin fibers. The fibers were characterized by atomic force microscopy and optical microscopy, and fluorescence microscopy was used for the characterization of target binding to the MIP. The fibers show close to 100% recovery upon extraction and rebinding of the target molecule. The selectivity of the system has been demonstrated through competitive binding experiments with nonfluorescent analogues boc-L-phenylalanine and boc-D-phenylalanine.
我们描述了带有包埋分子印迹聚合物 (MIP) 纳米粒子的聚合物纳米纤维的制造，并研究了它们在基于荧光的生物传感器应用中的可能用途。MIP 印迹了荧光氨基酸衍生物丹磺酰基-L-苯丙氨酸。聚（聚乙烯醇）被用作 MIP 纳米粒子的载体，因为它是水溶性的并且可以纺成非常细的纤维。通过原子力显微镜和光学显微镜对纤维进行了表征，并使用荧光显微镜对 MIP 与目标分子的结合进行了表征。在提取和再结合目标分子后，纤维的回收率接近 100%。通过与非荧光类似物 Boc-L-苯丙氨酸和 Boc-D-苯丙氨酸的竞争结合实验证明了该系统的选择性。
