测定吸入 13CD2-甲醛后非人类灵长类动物鼻腔上皮和骨髓中的 N2-羟甲基-dG 加合物。
Determination of N2-hydroxymethyl-dG adducts in the nasal epithelium and bone marrow of nonhuman primates following 13CD2-formaldehyde inhalation exposure.
机构信息
Curriculum in Toxicology, University of North Carolina, Chapel Hill, North Carolina 27599-7431, United States.
出版信息
Chem Res Toxicol. 2011 Feb 18;24(2):162-4. doi: 10.1021/tx1004166. Epub 2011 Jan 11.
Abstract
The presence of endogenous and exogenous N(2)-hydroxymethyl-dG adducts in DNA from the nasal mucosa and bone marrow of cynomolgus macaques exposed to 1.9 and 6.1 ppm of [(13)CD(2)]-formaldehyde for 6 h a day for 2 consecutive days was investigated using a highly sensitive nano-UPLC-MS/MS method with a limit of detection of 20 amol. Both exogenous and endogenous adducts were readily detected and quantified in the nasal tissues of both exposure groups, with an exposure dependent increase in exogenous adducts observed. In contrast, only endogenous adducts were detectable in the bone marrow, even though ∼10 times more DNA was analyzed.
摘要
采用灵敏度极高的纳升超高效液相色谱-串联质谱法（nano-UPLC-MS/MS），检测限低至 20 飞摩尔，检测并定量分析了连续 2 天、每天 6 小时暴露于 1.9 和 6.1ppm[（13）CD2]-甲醛环境中的食蟹猴鼻黏膜和骨髓 DNA 中内源性和外源性 N（2）-羟甲基-dG 加合物的存在情况。在两个暴露组的鼻组织中，均可轻易检测到并定量分析出外源性和内源性加合物，且观察到外源性加合物的暴露依赖性增加。相比之下，尽管分析的骨髓 DNA 量多了约 10 倍，但仅可检测到内源性加合物。
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