枸杞多糖对大鼠坐骨神经横断后创伤性神经瘤形成及疼痛的影响
[Effects of lycium barbarum polysaccharide on formation of traumatic neuroma and pain after transection of sciatic nerve in rats].
作者信息
Fan Hongwei, Deng Chunrong, Fu Jian, Ding Lei, Yin Gang, Ma Yulin
机构信息
No.3 Department of Orthopedics, Clinical Medicine School, Ningxia Medical University, Yinchuan Ningxia 750004, PR China.
出版信息
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2010 Nov;24(11):1298-301.
OBJECTIVE
To investigate the effects of lycium barbarum polysaccharide (LBP) on the formation of traumatic neuroma and pain after transection of sciatic nerve in rats.
METHODS
Forty Sprague-Dawley (SD) rats, weighing 200-220 g, half male and half female, were allocated into 2 groups randomly: LBP group and control group (n = 20 per group). The right sciatic nerves were transected and 2 cm sciatic nerve were removed in all rats of the 2 groups. LBP were intraperitoneally injected in a volume of 10 mg/(kgd) in the LBP group, while the same volume normal saline (NS) in the control group for 28 days. The deficiency of toenail and toe were observed to estimate the autophagy of the operated limb. Light microscope and transmission electron microscope were used to observe the formation of traumatic neuroma after transection of sciatic nerve.
RESULTS
Autophagy was observed in 5 rats (25%) of LBP group and in 12 rats (60%) of control group at 4 weeks, showing significant difference (P < 0.05). Neuroma formed in 8 rats (40%) of LBP group and in 16 rats (80%) of control group, showing significant difference (P < 0.05). The observation of light microscope showed that there were unorganized growth cells in the neuroma, infiltrated muscle cells, the regeneration of axons and ensheathing cells to form small patch and funicular structure in the control group, while in the LBP group there were less proliferation of nerve fibers with a regular arrangement. Transmission electron microscope showed that there were lots of axons in nerve tumour, more fusoid fibroblasts, more collagen fiber, and hyperplasia and degenerated myelin sheath in the control group, while in the LBP group there were less myelin sheath in the proximal end of injuring nerves, less Schwann cells and fibroblasts, and sparsed collagen fibers.
CONCLUSION
LBP can inhibit autophagy and the formation of traumatic neuroma after transection of sciatic nerve in rats.
目的
探讨枸杞多糖（LBP）对大鼠坐骨神经横断后创伤性神经瘤形成及疼痛的影响。
方法
将40只体重200 - 220 g的Sprague-Dawley（SD）大鼠，雌雄各半，随机分为2组：LBP组和对照组（每组20只）。两组大鼠均切断右侧坐骨神经并切除2 cm坐骨神经。LBP组大鼠腹腔注射LBP，剂量为10 mg/(kg·d)，对照组注射等体积的生理盐水（NS），连续28天。观察趾甲和趾的缺失情况以评估手术肢体的自噬。采用光镜和透射电镜观察坐骨神经横断后创伤性神经瘤的形成。
结果
4周时，LBP组有5只大鼠（25%）出现自噬，对照组有12只大鼠（60%）出现自噬，差异有统计学意义（P < 0.05）。LBP组有8只大鼠（40%）形成神经瘤，对照组有16只大鼠（80%）形成神经瘤，差异有统计学意义（P < 0.05）。光镜观察显示，对照组神经瘤中有生长无序的细胞、浸润的肌细胞、轴突和包鞘细胞再生形成小片状和索状结构，而LBP组神经纤维增殖较少且排列规则。透射电镜显示，对照组神经瘤中有大量轴突、较多梭形成纤维细胞、较多胶原纤维以及增生和退变的髓鞘，而LBP组损伤神经近端的髓鞘较少、施万细胞和成纤维细胞较少且胶原纤维稀疏。
结论
LBP可抑制大鼠坐骨神经横断后自噬及创伤性神经瘤的形成。
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