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热应激白来航鸡中的碳酸酐酶及其同工酶

Carbonic anhydrase and its isozymes in heat stressed white Leghorn hens.

作者信息

Gill S P, Gangwar P C

机构信息

Punjab Agricultural University, Department of Animal Science, Ludhiana, India.

出版信息

Arch Exp Veterinarmed. 1990;44(3):383-8.

PMID:2122871
Abstract

Experiments were conducted to study the effect of heat stress on uterine tissue carbonic anhydrase levels and their isozyme patterns in relation to production of no- and thin-shelled eggs. Birds exposed to naturally occurring heat stress had lower enzyme levels in their uteri than birds kept in an air-conditioned pen. 4 isozyme bands of carbonic anhydrase were visible in uterine tissue homogenate from both groups of birds. Isozymes were suppressed in heat-stressed birds, as was evident from staining intensities of the bands. This provided evidence to the effect that heat stress not only decreased the total quantity of carbonic anhydrase in the uteri of the hens but also suppressed its isozyme fractions, leading to greater production of no-shell and thin-shelled eggs.

摘要

进行实验以研究热应激对子宫组织碳酸酐酶水平及其同工酶模式的影响,以及与无壳蛋和薄壳蛋生产的关系。暴露于自然热应激的禽类子宫中的酶水平低于饲养在空调鸡舍中的禽类。两组禽类子宫组织匀浆中均可见4条碳酸酐酶同工酶带。从条带的染色强度可以明显看出,热应激禽类的同工酶受到抑制。这证明热应激不仅降低了母鸡子宫中碳酸酐酶的总量,还抑制了其同工酶组分,导致无壳蛋和薄壳蛋的产量增加。

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