在一株对疏水性药物耐药的人结肠癌细胞系中，多药耐药（mdr）基因表达增加，药物蓄积减少。
Increased mdr gene expression and decreased drug accumulation in a human colonic cancer cell line resistant to hydrophobic drug.
作者信息
Chao C C, Ma C M, Cheng P W, Lin-Chao S
机构信息
Tumor Biology Laboratory, Department of Biochemistry, Chang Gung Medical College, Taiwan, Republic of China.
出版信息
Biochem Biophys Res Commun. 1990 Oct 30;172(2):842-9. doi: 10.1016/0006-291x(90)90752-9.
An adriamycin-resistant human colonic cancer cell line was characterized. This clone exhibits the classical multidrug resistance (MDR) phenotype, being cross-resistant to hydrophobic drugs such as colchicine, and vinblastine. In contrast, this clone shows a normal response to DNA-damaging agents. The appearance of MDR in these cells was linked to a decreased accumulation of the drug [3H]colchicine as compared to the drug-sensitive cells. This MDR line expressed 80-100 fold increased levels of the specific 4.5-kb mdr mRNA, and a gene amplification. Our results indicate that MDR in human colonic cancer cells can result from increased expression of at least one member of the mdr gene family.
对一种耐阿霉素的人结肠癌细胞系进行了特性分析。该克隆呈现出典型的多药耐药（MDR）表型，对诸如秋水仙碱和长春碱等疏水药物具有交叉耐药性。相比之下，该克隆对DNA损伤剂表现出正常反应。与药物敏感细胞相比，这些细胞中MDR的出现与[3H]秋水仙碱药物积累的减少有关。该MDR细胞系中特异性4.5-kb mdr mRNA的表达水平增加了80至100倍，且存在基因扩增。我们的结果表明，人结肠癌细胞中的MDR可能是由于mdr基因家族中至少一个成员的表达增加所致。
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