Garbaccio S G, Cataldi A A
Instituto de Patobiología, CICVyA, Instituto Nacional de Tecnología Agropecuaria, Hurlingham, Argentina.
Rev Argent Microbiol. 2010 Oct-Dec;42(4):247-53. doi: 10.1590/S0325-75412010000400002.
Tuberculosis is one of the most important infectious diseases worldwide. Mycobacterium bovis is the causative agent of bovine tuberculosis (BTB), an important animal pathogen with public health implications as it is a zoonosis. Currently, the diagnosis of BTB is based on the caudal fold test of the Tuberculin Skin Test (TST). Post-mortem bacterial culture is carried out to confirm the diagnosis, and then specific biochemical tests are performed for the characterization of the etiologic agent. Culture takes at least 4 to 8 weeks to develop. The diagnosis by molecular tests such as PCR can provide fast and reliable results, significantly decreasing the time of confirmation (from two months to two days), thus allowing the possibility of taking control actions to prevent the spread of the disease in herds. In this work the use of an immunomagnetic separation capture followed by PCR (IMS-PCR) based on the IS6110 element showed a detection threshold corresponding to 10 CFU in M. bovis-spiked PBS. In the case of infected bovine fresh tissues, after five replicates, the minimum value of detection was 1000 CFU in 100% of the trials (5/5). This paper attempts to provide a sensitive, rapid and specific technique for the diagnosis of bovine tuberculosis, and opens up the possibility of a direct application in the control and eradication of this cattle disease.
结核病是全球最重要的传染病之一。牛分枝杆菌是牛结核病（BTB）的病原体，它是一种重要的动物病原体，由于是人畜共患病，对公共卫生有影响。目前，牛结核病的诊断基于结核菌素皮肤试验（TST）的尾褶试验。进行死后细菌培养以确诊，然后进行特定的生化试验以鉴定病原体。培养至少需要4至8周才能得出结果。通过PCR等分子检测进行诊断可以提供快速可靠的结果，显著缩短确诊时间（从两个月缩短到两天），从而有可能采取控制措施防止疾病在畜群中传播。在这项工作中，基于IS6110元件的免疫磁珠分离捕获后进行PCR（IMS-PCR）在加标牛分枝杆菌的磷酸盐缓冲盐水中显示出相当于10 CFU的检测阈值。对于感染的牛新鲜组织，经过五次重复试验后，在100%的试验（5/5）中检测最小值为1000 CFU。本文试图提供一种灵敏、快速且特异的牛结核病诊断技术，并为直接应用于控制和根除这种牛病开辟了可能性。
