Wei Su-Zhen, Liu Jun-Xin
Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
Huan Jing Ke Xue. 2010 Oct;31(10):2398-404.
In order to reveal the characteristics of microbial community in biohydrogen production from alkali pretreated sludge, the biohydrogen fermentation was conducted under acidic condition (pH 5) and alkali condition (pH 11) using alkali pretreated sludge from three wastewater treatment plants with different process, respectively. The results indicate that, although the sludge from different sources, protein is main component in the soluble organic matter released from the three sludge samples by the alkali pretreatment and carbohydrate is only 15% -16% of protein. A high hydrogen yield occurs at the condition of initial pH 11, a maximal hydrogen yield of up to 31.9 mL/g, while the hydrogen yield decreases and hydrogen consumption occurs at the condition of initial pH 5. The analysis using PCR-DGGE technique based on 16S rDNA sequences with the universal primers (F338GC and R534) show that the microbial community in the biohydrogen production process from various sludge structure is significant different, and the microbial population appear a phenomenon of turnover growth and decline and the amount of dominant microbial community present a increasing trend.
为揭示碱预处理污泥产氢过程中微生物群落的特征，分别以来自三个不同工艺的污水处理厂的碱预处理污泥为原料，在酸性条件（pH 5）和碱性条件（pH 11）下进行生物制氢发酵。结果表明，尽管污泥来源不同，但碱预处理从三种污泥样品中释放出的可溶性有机物中蛋白质是主要成分，碳水化合物仅为蛋白质的15% - 16%。在初始pH 11的条件下产氢量较高，最大产氢量可达31.9 mL/g，而在初始pH 5的条件下产氢量降低且出现氢气消耗。使用基于16S rDNA序列的通用引物（F338GC和R534）通过PCR - DGGE技术分析表明，不同污泥结构的生物制氢过程中微生物群落存在显著差异，微生物种群出现交替增长和下降的现象，优势微生物群落数量呈增加趋势。
