Xie Bo, Guo Liang, Li Xiao-ming, Yang Qi, Zhang Yu, Yang Yong-lin, Zeng Guang-ming, Liu Jing-jin, Yang Zhi-qiang
College of Environmental Science and Engineering, Hunan University, Changsha 410008, China.
Huan Jing Ke Xue. 2008 Apr;29(4):996-1001.
Batch tests of anaerobic fermentative hydrogen production by Pseudomonas sp. GL1 were investigated using sterilization, microwave and ultrasonication pretreated sludge as substrate. The profiles of soluble COD, protein, carbohydrate and pH value during the fermentation process were monitored. The results showed that only hydrogen and carbon dioxide were produced and methane was not observed during the process. A maximal hydrogen yield (30.07 mL x g(-1)) and bio-hydrogen content (81.45%) were obtained from the sterilization pretreated sludge run. The shortest lag time for hydrogen production was in ultrasonication pretreated sludge run (3 h), while the longest one was in sterilization pretreated sludge run (15 h), and the medial one was in microwave pretreated sludge run (12 h). It was found that the changes of sludge substrates (soluble COD, protein, carbohydrate and pH value) were various with different pretreated sludge during the fermentation process, especially in the sterilization sludge run, which implied that the pretreatment method could affect substrate utilization by Pseudomonas sp. GL1.
以经过灭菌、微波和超声预处理的污泥为底物，对假单胞菌GL1厌氧发酵产氢进行了批次试验。监测了发酵过程中可溶性化学需氧量、蛋白质、碳水化合物和pH值的变化情况。结果表明，该过程中仅产生氢气和二氧化碳，未观察到甲烷生成。采用经过灭菌预处理的污泥进行试验时，获得了最大产氢量（30.07 mL x g(-1)）和生物氢含量（81.45%）。产氢的最短延滞期出现在经过超声预处理的污泥试验中（3小时），最长的出现在经过灭菌预处理的污泥试验中（15小时），中间的出现在经过微波预处理的污泥试验中（12小时）。研究发现，在发酵过程中，不同预处理污泥的污泥底物（可溶性化学需氧量、蛋白质、碳水化合物和pH值）变化各异，尤其是在经过灭菌处理的污泥试验中，这表明预处理方法会影响假单胞菌GL1对底物的利用。
