正常和营养不良的仓鼠及小鼠组织中γ-谷氨酰转肽酶活性的比较。

Comparison of gamma-glutamyl transpeptidase activity in tissues of normal and dystrophic hamsters and mice.

作者信息

Neerunjun J S, Dubowitz V

出版信息

J Neurol Sci. 1977 Sep;33(3):335-40. doi: 10.1016/0022-510x(77)90130-7.

Abstract

The activity of gamma-glutamyl transpeptidase (gamma-GT), a membrane-bound enzyme, was assayed by a sensitive fluorometric method in the brain, heart, kidney, liver, skeletal muscle and serum of normal and dystrophic hamsters and mice. Normal and dystrophic hamsters were of the golden brown and BIO 14.6 line respectively. Both normal and dystrophic mice were of the C57 BL/6J -dy2j strain. Kidneys of both hamsters and mice contained the highest enzyme activity followed by the brain, skeletal muscle, liver and heart. Gamma-GT activity was elevated in dystrophic skeletal muscles but decreased in the dystrophic kidneys and sera. In the brain, heart and liver of dystrophic animals from both species enzyme activity was similar to that in normals.

摘要

γ-谷氨酰转肽酶（γ-GT）是一种膜结合酶，采用灵敏的荧光法对正常和营养不良的仓鼠及小鼠的脑、心脏、肾脏、肝脏、骨骼肌和血清中的该酶活性进行了测定。正常和营养不良的仓鼠分别为金棕色品系和BIO 14.6品系。正常和营养不良的小鼠均为C57 BL/6J -dy2j品系。仓鼠和小鼠的肾脏中该酶活性最高，其次是脑、骨骼肌、肝脏和心脏。营养不良的骨骼肌中γ-GT活性升高，但营养不良的肾脏和血清中该酶活性降低。在这两个物种的营养不良动物的脑、心脏和肝脏中，酶活性与正常动物相似。