Waddell Jack N, Mullen Douglas G, Orr Bradford G, Banaszak Holl Mark M, Sander Leonard M
Department of Mathematics, University of Michigan, Ann Arbor, Michigan 48109, USA.
Phys Rev E Stat Nonlin Soft Matter Phys. 2010 Sep;82(3 Pt 2):036108. doi: 10.1103/PhysRevE.82.036108. Epub 2010 Sep 15.
Nanoparticles with multiple ligands have been proposed for use in nanomedicine. The multiple targeting ligands on each nanoparticle can bind to several locations on a cell surface facilitating both drug targeting and uptake. Experiments show that the distribution of conjugated ligands is unexpectedly broad, and the desorption rate appears to depend exponentially upon the mean number of attached ligands. These two findings are explained with a model in which ligands conjugate to the nanoparticle with a positive cooperativity of ≈4 kT , and that nanoparticles bound to a surface by multiple bonds are permanently affixed. This drives new analysis of the data, which confirms that there is only one time constant for desorption, that of a nanoparticle bound to the surface by a single bond.
具有多种配体的纳米颗粒已被提议用于纳米医学。每个纳米颗粒上的多个靶向配体可以结合到细胞表面的多个位置，从而促进药物靶向和摄取。实验表明，共轭配体的分布出乎意料地广泛，并且解吸速率似乎与附着配体的平均数呈指数关系。这两个发现可以用一个模型来解释，在该模型中，配体以约4kT的正协同性与纳米颗粒共轭，并且通过多个键与表面结合的纳米颗粒被永久固定。这推动了对数据的新分析，证实解吸只有一个时间常数，即通过单键与表面结合的纳米颗粒的时间常数。
