Richards Erin, Li Shihui, Chen Niancao, Battig Mark R, Wang Yong
Department of Biomedical Engineering, The Pennsylvania State University , University Park, Pennsylvania 16802-6804, United States.
Biomacromolecules. 2014 Dec 8;15(12):4561-9. doi: 10.1021/bm501347s. Epub 2014 Nov 3.
Surfaces functionalized with affinity ligands have been widely studied for applications such as biological separations and cell regulation. While individual ligands can be directly conjugated onto a surface, it is often important to conjugate polyvalent ligands onto the surface to enhance ligand display. This study was aimed at exploring a method for surface functionalization via polymerization of affinity ligands, which was achieved through ligand hybridization with DNA polymers protruding from the surface. The surface with polyvalent ligands was evaluated via aptamer-mediated cell binding. The results show that this surface bound target cells more effectively than a surface directly functionalized with individual ligands in situations with either equal amounts of ligand display or equal amounts of surface reaction sites. Therefore, this study has demonstrated a new strategy for surface functionalization to enhance ligand display and cell binding. This strategy may find broad applications in settings where surface area is limited or the surface of a material does not possess sufficient reaction sites.
用亲和配体功能化的表面已被广泛研究用于生物分离和细胞调控等应用。虽然单个配体可以直接连接到表面上，但将多价配体连接到表面上以增强配体展示通常很重要。本研究旨在探索一种通过亲和配体聚合进行表面功能化的方法，这是通过与从表面突出的DNA聚合物进行配体杂交来实现的。通过适体介导的细胞结合对具有多价配体的表面进行了评估。结果表明，在配体展示量相等或表面反应位点数量相等的情况下，该表面比直接用单个配体功能化的表面更有效地结合靶细胞。因此，本研究展示了一种用于表面功能化以增强配体展示和细胞结合的新策略。这种策略可能在表面积有限或材料表面没有足够反应位点的情况下找到广泛的应用。
