GWC Technologies Inc., Madison, WI 53719, USA.
Biotechniques. 2011 Jan;50(1):32-40. doi: 10.2144/000113569.
Surface plasmon resonance (SPR) is a label-free detection method by which molecular interactions may be analyzed on a surface. Binding data are collected in real time, allowing the determination of interaction kinetics. SPR imaging (SPRi), the focus of this review, improves upon the efficiency of SPR by facilitating analysis of multiple interactions simultaneously. Here we summarize the principles of SPRi, provide examples of how SPRi arrays can be fabricated, and illustrate the utility of SPRi through example applications from the fields of proteomics, genomics and bioengineering.
表面等离子体共振（SPR）是一种无需标记的检测方法，可以在表面上分析分子相互作用。结合数据实时采集，可确定相互作用动力学。本文主要关注 SPR 成像（SPRi），通过同时促进多种相互作用的分析，提高 SPR 的效率。我们总结了 SPRi 的原理，提供了 SPRi 阵列的制作实例，并通过蛋白质组学、基因组学和生物工程领域的应用实例说明了 SPRi 的实用性。
