Honstra G, van Houwelingen A C, Kivits G A, Fischer S, Uedelhoven W
Department of Human Biology, Limburg University, Maastricht, The Netherlands.
Prostaglandins. 1990 Sep;40(3):311-29. doi: 10.1016/0090-6980(90)90018-q.
Two groups of 40 volunteers were given a dietary supplement consisting of 135 g of mackerel or meat (control) paste per day for 6 weeks. Compliance was about 80% in both groups and the daily intake of 20:5(n-3) and 22:6(n-3) from the mackerel supplement was about 1.3 and 2.3 g, respectively. In collagen-activated platelet rich plasma, the potency of blood platelet to produce HHT from arachidonic acid (AA) clearly reduced in the mackerel group, whereas the formation of HHTE from timnodonic acid (TA) increased slightly. Changes in the formation of HHT and HHTE, measured by HPLC, correlated significantly with those of TxB2 and TxB3, respectively, measured by GC/MS. Changes in the formation of the lipoxygenase products HETE (ex AA) and HEPE (ex TA) were qualitatively similar to that seen for the cyclo-oxygenase products, but quantitatively the responses were smaller. Formation of ir TxB2 in clotting blood significantly reduced in the mackerel group. In collagen-activated, citrated whole blood, TxB2 formation tended to be reduced in the mackerel-supplemented volunteers. Mackerel consumption was associated with the formation of considerable amounts of PGI3, as judged from the appearance of 2,3-dinor-delta 17-6-keto-PGF1 alpha in urine. The amount of the major metabolite of PGI2, 2,3-dinor-6-keto-PGF1 alpha was not reduced, or even increased. The daily amount of tetranor prostaglandin metabolites in the urine did not change significantly, which indicates that mackerel supplementation did not alter the formation of prostaglandins E and F.
两组各40名志愿者每天服用一种膳食补充剂，其中一组服用135克鲭鱼酱，另一组服用肉类（对照）酱，持续6周。两组的依从性约为80%，从鲭鱼补充剂中每日摄入的20:5(n-3)和22:6(n-3)分别约为1.3克和2.3克。在胶原激活的富血小板血浆中，鲭鱼组血小板从花生四烯酸（AA）产生11-羟基-5,8,12,14-二十碳四烯酸（HHT）的能力明显降低，而从二十碳五烯酸（TA）产生11-羟基-5,8,11,14,17-二十碳五烯酸（HHTE）的能力略有增加。通过高效液相色谱法（HPLC）测定的HHT和HHTE形成的变化，分别与通过气相色谱-质谱联用仪（GC/MS）测定的血栓素B2（TxB2）和血栓素B3（TxB3）的变化显著相关。脂氧合酶产物5-羟基-6,8,11,14-二十碳四烯酸（HETE，源自AA）和5-羟基-6,8,11,14,17-二十碳五烯酸（HEPE，源自TA）形成的变化在质量上与环氧化酶产物相似，但在数量上反应较小。鲭鱼组凝血血液中免疫反应性TxB2的形成显著降低。在胶原激活的枸橼酸化全血中，补充鲭鱼的志愿者中TxB2的形成有降低的趋势。从尿液中出现2,3-二去甲-δ17-6-酮-前列环素F1α（2,3-dinor-delta 17-6-keto-PGF1 alpha）判断，食用鲭鱼与大量前列环素I3（PGI3）的形成有关。前列环素I2（PGI2）的主要代谢产物2,3-二去甲-6-酮-前列环素F1α（2,3-dinor-6-keto-PGF1 alpha）的量没有减少，甚至有所增加。尿液中四去甲前列腺素代谢产物的每日量没有显著变化，这表明补充鲭鱼不会改变前列腺素E和F的形成。
