用于定量检测人γ-γ-烯醇化酶（NSE）的酶免疫测定法
[Enzyme immunoassays for the quantification of human gamma-gamma-enolase (NSE)].
作者信息
Gross J, Zinsmeyer J, Pohle R, Moller R, Schmechta H, Porstmann B, Porstmann T, Brux B
机构信息
Institut für Pathologische und Klinische Biochemie, Bereichs Medizin (Charité) der Humboldt-Universität zu Berlin.
出版信息
Z Med Lab Diagn. 1990;31(5):258-65.
A direct two-site binding assay on the basis of antibodies from sheep for the quantification of human gamma-gamma enolase is described. The antibody was produced by immunization with human NSE coupled to horse spleen ferritin. The assay shows two feature: a decreased reactivity with NSE from rat and NSE from human serum in spite of 100% recovery of purified human brain NSE. The sheep antibody seems to react with epitopes less accessible on the rat NSE and on the NSE of human serum. The assay is characterized by gamma-gamma enolase specificity, a high sensitivity (2 pg) and a precision of CV = 3-7%.
描述了一种基于绵羊抗体的直接双位点结合测定法，用于定量人γ-γ烯醇化酶。该抗体通过用人NSE与马脾铁蛋白偶联免疫制备。该测定法有两个特点：尽管纯化的人脑中NSE回收率为100%，但与大鼠NSE和人血清NSE的反应性降低。绵羊抗体似乎与大鼠NSE和人血清NSE上较难接近的表位发生反应。该测定法具有γ-γ烯醇化酶特异性、高灵敏度（2 pg）和变异系数（CV）为3-7%的精密度。
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