Graduate School of Integrated Arts and Sciences, Hiroshima University, Kagamiyama 1-7-1, Higashi-Hiroshima, Hiroshima 739-8521, Japan.
Mol Genet Genomics. 2011 Mar;285(3):219-24. doi: 10.1007/s00438-010-0599-3. Epub 2011 Jan 15.
To investigate the recent transposition activity of T2 family miniature inverted-repeat transposable elements (MITEs) in Xenopus tropicalis (Western clawed frog), we analyzed the intraspecific polymorphisms associated with MITE insertion in X. tropicalis for three subfamilies of the T2 family (T2-A1, T2-C, and T2-E). A high frequency of MITE-insertion polymorphisms was observed at the T2-A1 (50%) and T2-C insertion loci (60%), but none were noted at the T2-E insertion locus (0%). Analyses of the collected data indicated that members of the T2-A1 and T2-C subfamilies may be currently active in the host species. Identification of these active transpositions will help us in understanding the mechanisms underlying the long-term survival (over several tens of millions of years) of the T2-A1 and T2-C subfamilies.
为了研究 T2 家族微型反转录转座子（MITE）在 Xenopus tropicalis（西部爪蟾）中的近期转座活性，我们分析了与 X. tropicalis 中 MITE 插入相关的种内多态性，这些 MITE 插入来自 T2 家族的三个亚家族（T2-A1、T2-C 和 T2-E）。在 T2-A1（50%）和 T2-C 插入位点（60%）观察到了高频 MITE-插入多态性，但在 T2-E 插入位点（0%）未观察到。对收集到的数据的分析表明，T2-A1 和 T2-C 亚家族的成员可能在宿主物种中活跃。鉴定这些活跃的转座将有助于我们理解 T2-A1 和 T2-C 亚家族在数千万年的长期生存（over several tens of millions of years）中的机制。
