LSRE-Laboratory of Separation and Reaction Engineering, Associate Laboratory LSRE/LCM, Department of Chemical Engineering, University of Porto, Rua, Portugal.
Appl Biochem Biotechnol. 2011 Jun;164(4):524-33. doi: 10.1007/s12010-010-9154-2. Epub 2011 Jan 15.
The activity and stability of laccase and their kinetic mechanisms in water soluble ionic liquids (ILs): 1-butyl-3-methyl imidazolium chloride [C(4)mim][Cl], 1-octyl-3-methyl imidazolium chloride [C(8)mim][Cl], and 1-decyl-3-methyl imidazolium chloride [C(10)mim][Cl] were investigated. The results show that an IL concentration up to 10% is satisfactory for initial laccase activity at pH 9.0. The laccase stability was well maintained in [C(4)mim][Cl] IL when compared to the control. The inactivation of laccase increases with the length of the alkyl chain in the IL: [C(10)mim][Cl] > [C(8)mim][Cl] > [C(4)mim][Cl]. The kinetic studies in the presence of ABTS as substrate allowed calculating the Michaelis-Menten parameters. Among the ILs, [C(4)mim][Cl] was the suitable choice attending to laccase activity and stability. Alkyl chains in the ions of ILs have a deactivating effect on laccase, which increases strongly with the length of the alkyl chain.
研究了水溶性离子液体（ILs）中的漆酶的活性和稳定性及其动力学机制：1-丁基-3-甲基咪唑氯[C（4）mim][Cl]、1-辛基-3-甲基咪唑氯[C（8）mim][Cl]和 1-癸基-3-甲基咪唑氯[C（10）mim][Cl]。结果表明，在 pH 9.0 时，IL 浓度高达 10%即可满足初始漆酶活性的要求。与对照相比，漆酶在[C（4）mim][Cl] IL 中保持了良好的稳定性。当 IL 中的烷基链长度增加时，漆酶的失活增加：[C（10）mim][Cl]>[C（8）mim][Cl]>[C（4）mim][Cl]。以 ABTS 为底物的动力学研究允许计算米氏-门捷列夫参数。在 ILs 中，[C（4）mim][Cl]是根据漆酶活性和稳定性选择的合适选择。IL 离子中的烷基链对漆酶具有失活作用，随着烷基链长度的增加而强烈增加。
