Fowler P A, Messinis I E, Templeton A A
Department of Obstetrics and Gynaecology, University of Aberdeen, Foresterhill, UK.
J Reprod Fertil. 1990 Nov;90(2):587-94. doi: 10.1530/jrf.0.0900587.
It has been suggested that in superovulated women the endogenous LH surge is attenuated by a non-steroidal factor, called gonadotrophin surge-attenuating factor (GnSAF), which reduces gonadotrophin secretion in response to LHRH. To determine whether human follicular fluid (hFF) from superovulated women contains GnSAF activity, the secretion of LH and FSH by cultured sheep pituitaries was studied. After charcoal extraction of steroids, hFF was treated by heparin/Sepharose chromatography, which reversibly binds inhibin. The effects of whole hFF and the bound and unbound fractions on basal and LHRH-induced gonadotrophin secretion were then assessed. Steroid-free hFF significantly reduced basal FSH, but not basal LH, secretion, and significantly attenuated the LH and FSH responses to LHRH. The bound (inhibin) fraction significantly decreased both basal and LHRH-induced FSH secretion but did not affect LH release. The unbound fraction had no effect on basal LH or FSH secretion, but significantly attenuated LHRH-induced secretion of both LH and FSH. We conclude that the unbound fraction of hFF from superovulated women contains GnSAF. It has been demonstrated that GnSAF is a non-steroidal factor and its activity is distinct from that of inhibin.
有人提出，在超排卵的女性中，内源性促黄体生成素（LH）峰被一种非甾体因子减弱，这种因子称为促性腺激素峰减弱因子（GnSAF），它会减少对促性腺激素释放激素（LHRH）的促性腺激素分泌。为了确定超排卵女性的人卵泡液（hFF）是否含有GnSAF活性，研究了培养的绵羊垂体分泌LH和促卵泡生成素（FSH）的情况。在用活性炭提取类固醇后，hFF通过肝素/琼脂糖凝胶层析进行处理，该层析可与抑制素可逆性结合。然后评估了完整hFF以及结合和未结合部分对基础和LHRH诱导的促性腺激素分泌的影响。无类固醇的hFF显著降低基础FSH分泌，但不降低基础LH分泌，并显著减弱LH和FSH对LHRH的反应。结合（抑制素）部分显著降低基础和LHRH诱导的FSH分泌，但不影响LH释放。未结合部分对基础LH或FSH分泌无影响，但显著减弱LHRH诱导的LH和FSH分泌。我们得出结论，超排卵女性hFF的未结合部分含有GnSAF。已经证明GnSAF是一种非甾体因子，其活性与抑制素不同。
