[不同穴位电针对大鼠结肠扩张后血压及自主神经系统变化的影响]

[Effects of electroacupuncture of different acupoints on changes of blood pressure and autonomic nerve system after colorectal distension in rats].

作者信息

Chen Shu-Ping, Gao Yong-Hui, Yu Xiao-Chun, Liu Jun-Ling

机构信息

Institute of Acu-moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China.

出版信息

Zhen Ci Yan Jiu. 2010 Oct;35(5):335-41.

DOI:

PMID:21235060

Abstract

OBJECTIVE

To observe the influence of electroacupuncture (EA) of different acupoints on changes of mean arterial pressure (MAP), heart rate (HR) and heart rate variability (HRV) in colorectal distension (CRD) rats, so as to analyze the specificity of actions of acupoints in relieving visceral pain and regulating activities of the autonomic nerve system.

METHODS

Forty-five Wistar rats were randomized into control, Zusanli (ST 36), non-acupoint, Neiguan (PC 6) and Taichong (LR 3) groups (n = 9/group). Under anesthesia, CRD was given to the rats by using an aerostat for 5 min. EA (2 Hz/15 Hz, 2 mA) was applied to bilateral ST 36, non-acupoint (1.0 cm lateral to ST 36), PC 6 and LR 3 for 15 min, respectively. Electrocardiogram of the cervico-chest lead was recorded by using a bioelectric amplifier, and MAP recorded by using a pressure transducer and an amplifier. Low frequency and high frequency of HRV were analyzed by Chart 5.0.

RESULTS

Following CRD, the HR, MAP, LF and LF/HF levels increased significantly in all the 5 groups (P < 0.05), while HF had no apparent changes (P > 0.05). Compared with the control group, 5 min and 15 min after EA ,and 10 min after ceasing EA, MAP values of ST 36 and PC 6 groups were decreased obviously (P < 0.05). The HR values of ST 36 and PC 6 groups at 15 min after EA and 10 min after ceasing EA were decreased markedly in comparison with those of each of the other groups during CRD (P < 0.05). The LF levels of both ST 36 and PC 6 groups at 5 min after EA, and those of ST 36, PC 6 and LR 3 at 15 min after EA were significantly lower than those of control group (P < 0.05). LF/HF levels of ST 36 and PC 6 at 10 min after ceasing EA were obviously lower than those of control group (P < 0.05). No significant differences were found among ST 36, non-acupoint, PC 6 and LR 3 groups in HR, LF and LF/HF (P > 0.05).

CONCLUSION

EA of ST 36 and PC 6 can suppress CRD-induced increase of MAP, HR and LF/HF, suggesting beneficial effects of EA in relieving visceral pain and mediating autonomic nerve system. The aforementioned effects of EA of LR 3 and non-acupoint are not obvious.

摘要

目的

观察电针不同穴位对结直肠扩张（CRD）大鼠平均动脉压（MAP）、心率（HR）及心率变异性（HRV）变化的影响，以分析穴位在缓解内脏痛及调节自主神经系统活动方面的作用特异性。

方法

45只Wistar大鼠随机分为对照组、足三里（ST 36）组、非穴位组、内关（PC 6）组和太冲（LR 3）组（每组n = 9）。麻醉下，用气体调节器对大鼠进行CRD 5分钟。分别将电针（2 Hz/15 Hz，2 mA）施加于双侧ST 36、非穴位（ST 36旁开1.0 cm）、PC 6和LR 3，持续15分钟。用生物电放大器记录颈胸导联心电图，用压力传感器和放大器记录MAP。用Chart 5.0分析HRV的低频和高频成分。

结果

CRD后，5组大鼠的HR、MAP、LF及LF/HF水平均显著升高（P < 0.05），而HF无明显变化（P > 0.05）。与对照组相比，电针后5分钟、15分钟及停针后10分钟，ST 36组和PC 6组的MAP值明显降低（P < 0.05）。电针后15分钟及停针后10分钟，ST 36组和PC 6组的HR值与CRD期间其他各组相比明显降低（P < 0.05）。电针后5分钟，ST 36组和PC 6组的LF水平，以及电针后15分钟，ST 36组、PC 6组和LR 3组的LF水平均显著低于对照组（P < 0.05）。停针后10分钟，ST 36组和PC 6组的LF/HF水平明显低于对照组（P < 0.05）。ST 36组、非穴位组、PC 6组和LR 3组在HR、LF及LF/HF方面无显著差异（P > 0.05）。