Castellazzi M, Dangy J P, Mechta F, Hirai S, Yaniv M, Samarut J, Lassailly A, Brun G
Laboratoire de Biologie Moléculaire et Cellulaire, CNRS-UMR 49, Ecole Normale Supérieure, Lyon, France.
Oncogene. 1990 Oct;5(10):1541-7.
The coding sequences of avian (quail) or murine c-jun proto-oncogenes were introduced into a non-defective retroviral vector derived from Rous sarcoma virus (RSV) in which c-jun replaces v-src. Primary avian fibroblasts chronically infected with either one of these viruses exhibit some phenotypic traits characteristic of RSV-transformed cells, including sustained growth in low serum medium and ability to develop colonies from single cells in agar, even though they are still of normal morphology and contact inhibited. This altered growth control correlates with enhanced AP1-specific DNA binding activity as well as with higher levels of c-Jun products. Unexpectedly, repression of the endogenous c-Jun product is observed in cells overexpressing murine c-Jun. Cells expressing the avian and the murine c-Jun products display qualitatively similar phenotypes; nevertheless, for every transformed trait considered, the murine c-jun seemed more potent than its quail homologue. These data suggest that the avian or murine c-jun proto-oncogenes may trigger a subset of the 'transforming functions' normally induced by v-src, and which are more specifically related to growth in low serum and in the absence of solid support.
将禽（鹌鹑）或鼠源c-jun原癌基因的编码序列导入源自劳斯肉瘤病毒（RSV）的无缺陷逆转录病毒载体中，其中c-jun取代了v-src。用这两种病毒中的任何一种长期感染的原代禽成纤维细胞表现出一些RSV转化细胞特有的表型特征，包括在低血清培养基中持续生长以及在琼脂中从单细胞形成集落的能力，尽管它们的形态仍然正常且具有接触抑制。这种生长控制的改变与增强的AP1特异性DNA结合活性以及更高水平的c-Jun产物相关。出乎意料的是，在过表达鼠源c-Jun的细胞中观察到内源性c-Jun产物的抑制。表达禽源和鼠源c-Jun产物的细胞表现出定性相似的表型；然而，对于所考虑的每一个转化特征，鼠源c-jun似乎比其鹌鹑同源物更有效。这些数据表明，禽源或鼠源c-jun原癌基因可能触发了通常由v-src诱导的“转化功能”的一个子集，并且这些功能更具体地与低血清和无固体支持物条件下的生长相关。
