Salley S O, Song J Y, Whittlesey G C, Klein M D
Department of Chemical Engineering, Wayne State University, Detroit, MI.
ASAIO Trans. 1990 Jul-Sep;36(3):M486-90.
The potential use of carbonic anhydrase (CA), immobilized within membrane lungs, to accelerate the removal of CO2 from blood was investigated. Using a variation on the technique of Chang, 500 micrograms/mL of CA was encapsulated in 5-20 microns cellulose nitrate microcapsules which were then immobilized onto a 0.1 m2 silicone rubber membrane. Using an in vitro test circuit, 0.68 mmol/m2/min of CO2 was removed from venous blood through an untreated membrane at steady state. With the CA-immobilized membrane, CO2 removal was enhanced to 1.09 mmol/m2/min. This technique has the potential of greatly improving the CO2 efficiency of commercial membrane lungs for ECCOR.
研究了固定在膜肺中的碳酸酐酶（CA）加速从血液中去除二氧化碳的潜在用途。采用了一种对张的技术的改进方法，将500微克/毫升的CA包裹在5至20微米的硝酸纤维素微胶囊中，然后将这些微胶囊固定在0.1平方米的硅橡胶膜上。使用体外测试回路，在稳态下，通过未处理的膜从静脉血中去除的二氧化碳量为0.68毫摩尔/平方米/分钟。使用固定有CA的膜时，二氧化碳去除量提高到了1.09毫摩尔/平方米/分钟。该技术具有极大提高用于体外心肺复苏（ECCOR）的商用膜肺的二氧化碳去除效率的潜力。
