Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology (Ministry of Education), Department of Chemistry, Tsinghua University, Beijing 100084, PR China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2011 Feb 1;879(3-4):296-8. doi: 10.1016/j.jchromb.2010.12.015. Epub 2010 Dec 28.
A simple, rapid, precise and eco-friendly ion chromatography (IC) method for the determination of hippuric acid (HA) in human urine was proposed in this paper. The separation was carried out an anion exchange column with 2.0 mmol L⁻¹ Na₂CO₃ + 2.0 mmol L⁻¹ NaHCO₃ as mobile phase at the flow-rate 0.7 mL min⁻¹. A suppressed conductivity detector was used and the detection limit was 1.0 μg L⁻¹ (S/N=3) for hippuric acid. The analysis time for one run was 30 min under the optimized IC condition. The recovery of hippuric acid was 93.2-98.0% while the relative standard deviation (RSD) was 1.4-2.3% by seven measurements.
本文提出了一种简单、快速、准确且环保的离子色谱法（IC）测定人尿液中马尿酸（HA）的方法。分离在阴离子交换柱上进行，以 2.0 mmol L⁻¹ Na₂CO₃ + 2.0 mmol L⁻¹ NaHCO₃ 为流动相，流速为 0.7 mL min⁻¹。使用抑制型电导检测器，马尿酸的检测限为 1.0 μg L⁻¹（S/N=3）。在优化的 IC 条件下，一次运行的分析时间为 30 min。马尿酸的回收率为 93.2-98.0%，7 次测量的相对标准偏差（RSD）为 1.4-2.3%。
