McDougall C J, Ngoi S S, Goldman I S, Godwin T, Felix J, DeCosse J J, Rigas B
Department of Medicine, Cornell University Medical College, New York, New York 10021.
Cancer Res. 1990 Dec 15;50(24):8023-7.
The expression of HLA class I and II antigens was studied by immunohistochemistry in (a) specimens of colon cancer from 25 patients, (b) normal colonic mucosa obtained 5-10 cm away from each tumor, and (c) colonic mucosa from 13 normal individuals. Thirteen of the tumor specimens had normal epithelium adjacent to the cancer, which thus served as an internal control. The expression of HLA class I antigens in colon cancer was dramatically reduced compared to control (P less than 0.0001): undetectable in 28%, diminished in 68%, normal in 4%. The expression of class II antigens was also reduced in cancer (P less than 0.0001 for all when compared to normal), being undetectable in most (HLA-DP 64%, HLA-DQ 72%, HLA-DR 68%). In 44% of the cancers all three HLA class II antigens were undetectable; in 92% at least one class II antigen was undetectable; and in 20% both class I and class II antigens were undetectable. No cancer specimen had a completely normal HLA phenotype. The expression of other surface antigens was preserved in cancer tissues and, therefore, loss of HLA antigens was not due to a nonspecific decline in surface molecules. When glands of normal mucosa immediately adjacent to cancer were compared to those of normal controls, significantly reduced expression of only HLA class I antigens (P = 0.0149) and HLA-DP (P = 0.034) was found. The expression of the HLA antigens in colonic mucosa remote from the cancer was no different from that of normal controls. Our data show extensive and significant reduction in the expression of HLA antigens in colon cancer; its potential relationship to immunosurveillance in cancer is discussed.
通过免疫组织化学方法研究了（a）25例结肠癌患者的标本、（b）距每个肿瘤5 - 10厘米处获取的正常结肠黏膜以及（c）13名正常个体的结肠黏膜中HLA I类和II类抗原的表达情况。13个肿瘤标本在癌旁有正常上皮，可作为内部对照。与对照组相比，结肠癌中HLA I类抗原的表达显著降低（P < 0.0001）：28%检测不到，68%减少，4%正常。II类抗原在癌组织中的表达也降低（与正常组织相比，所有情况P < 0.0001），大多数情况下检测不到（HLA - DP为64%，HLA - DQ为72%，HLA - DR为68%）。在44%的癌症中，所有三种HLA II类抗原均检测不到；92%的癌症中至少有一种II类抗原检测不到；20%的癌症中I类和II类抗原均检测不到。没有癌症标本具有完全正常的HLA表型。癌组织中其他表面抗原的表达得以保留，因此HLA抗原的缺失并非由于表面分子的非特异性下降。当将紧邻癌症的正常黏膜腺与正常对照的腺进行比较时，仅发现HLA I类抗原（P = 0.0149）和HLA - DP（P = 0.034）的表达显著降低。远离癌症的结肠黏膜中HLA抗原的表达与正常对照无异。我们的数据表明结肠癌中HLA抗原的表达广泛且显著降低；并讨论了其与癌症免疫监视的潜在关系。
