Takei Y, Kauffman F C, Misra U K, Yamanaka H, Thurman R G
Department of Pharmacology, UNC-CH 27599-7365.
Biochim Biophys Acta. 1990 Dec 6;1036(3):242-4. doi: 10.1016/0304-4165(90)90041-t.
Periportal and pericentral regions of the liver lobule were isolated from perfused rat liver using a micropunch and incubated in Krebs-Henseleit buffer (pH 7.6) containing 2% poly(ethylene glycol) in Eagle's basal medium, PMSF (50 micrograms/ml) and leupeptin (20 micrograms/ml) for 2 h at 25 degrees C under and O2/CO2 (95:5%) gas phase. Maximal rates of urea production from ammonium chloride were 96.4 +/- 8.7 and 32.8 +/- 5.4 mumol/g per h at 800 and 200 microM O2. Thus, urea synthesis was 2-3-times greater at high than low O2 tension in plugs from periportal and pericentral regions of the liver lobule.
使用微量打孔器从灌注的大鼠肝脏中分离出肝小叶的门周和中央周围区域，并在含有2%聚乙二醇的Eagle基础培养基、苯甲基磺酰氟（PMSF，50微克/毫升）和亮抑酶肽（20微克/毫升）的Krebs-Henseleit缓冲液（pH 7.6）中，于25℃、O₂/CO₂（95:5%）气相条件下孵育2小时。在800微摩尔和200微摩尔O₂浓度下，氯化铵生成尿素的最大速率分别为96.4±8.7和32.8±5.4微摩尔/克每小时。因此，在肝小叶门周和中央周围区域的组织块中，高氧张力下尿素合成比低氧张力时大2至3倍。
