Xu Jianfang, Zhou Caicun, Zhang Haiping, Su Bo, Tang Liang, Cheng Beiling, Qin Ping, Li Aiwu
Department of Medical Oncology, Shanghai Pulmonary Hospital, Shanghai 200433, P.R.China.
Zhongguo Fei Ai Za Zhi. 2004 Aug 20;7(4):336-8. doi: 10.3779/j.issn.1009-3419.2004.04.16.
To investigate the relationship between cytokine level and cancer cachexia on murine model, and to observe the effects of regular dose of indomethacin on cancer cachexia and survival of mice.
C57 mice bearing Lewis lung carcinoma were used to establish murine cancer cachexia model. The serum cytokine levels (IL-1, IL-6, TNF-α and IFN-γ) and body weight of the mice were measured at different time points before and after treatment with daily intraperioneal injection of either indomethacin (1 mg/kg) or saline.
The mice in cancer cachexia group had significantly higher serum levels of IL-1, IL-6 and TNF-α ( P < 0.05) and lower body weight ( P < 0.05) than those in healthy control group. Compared to saline treatment, indomethacin intervention apparently down regulated the levels of IL-1, IL-6 and TNF-α ( P < 0.05 ), and remarkably prolonged the survival of mice ( P < 0.05). No significant difference in IFN-γ level was observed between cancer cachexia and healthy control groups ( P > 0.05), as well as between indomethacin and saline groups ( P > 0.05).
The results suggest that serum IL-1, IL-6 and TNF-α are possibly associated with cancer cachexia, however, IFN-γ seems to be irrelevant. Indomethacin may potentially ameliorate cancer cachexia through down-regulating levels of cytokines.
在小鼠模型中研究细胞因子水平与癌症恶病质之间的关系，并观察常规剂量消炎痛对癌症恶病质及小鼠生存的影响。
采用荷Lewis肺癌的C57小鼠建立小鼠癌症恶病质模型。每日腹腔注射消炎痛（1mg/kg）或生理盐水，在治疗前后的不同时间点测量小鼠血清细胞因子水平（IL-1、IL-6、TNF-α和IFN-γ）及体重。
癌症恶病质组小鼠血清IL-1、IL-6和TNF-α水平显著高于健康对照组（P<0.05），体重低于健康对照组（P<0.05）。与生理盐水治疗相比，消炎痛干预明显下调了IL-1、IL-6和TNF-α水平（P<0.05），并显著延长了小鼠的生存期（P<0.05）。癌症恶病质组与健康对照组之间以及消炎痛组与生理盐水组之间的IFN-γ水平均无显著差异（P>0.05）。
结果表明，血清IL-1、IL-6和TNF-α可能与癌症恶病质有关，而IFN-γ似乎与之无关。消炎痛可能通过下调细胞因子水平来改善癌症恶病质。
