Wu Yan-Ling, Sun Shu-Ai, Wang Zhi-Ping, Li Hui-Qing, Xie Ke-Qin
Department of Epidemiology and Health Statistics, School of Public Health, Shandong University, Jinan 250012, China.
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2010 Dec;28(12):881-4.
To observe the effect of carbon disulfide (CS(2)) on the expression of matrix metalloproteinase (MMP)-2, MMP-9 in mouse embryo and uterus tissues and to explore the mechanism of embryo toxicity induced by CS(2).
At the phases of follicular development and embryonic implantation which was subdivided into early-implantation phase and late-implantation phase, mice were intraperitoneally exposed to CS(2) (the dosage was 631.4 mg/kg, and the volume was 0.1ml/10 g body weight) for 2 consecutive days. All indicators were got at the ninth day in gestation, and the expression of MMP-2 and MMP-9 in embryo and uterus tissues was analyzed by gelatin zymography.
The number of implanted embryos significantly decreased after exposure at late-implantation phase (16.000 ± 12.166) compared with those of the control (30.700 ± 5.599, P < 0.05). Expression of MMP-2 and MMP-9 in embryos declined obviously at the three reproductive phases (P < 0.01), and the levels of MMP-2 and MMP-9 expression in embryos at the phases of late-implantation phase (0.6837 ± 0.0929, 0.7309 ± 0.0822) and follicular development (0.6222 ± 0.0997, 0.7520 ± 0.1068) were much lower than those of the control (1.0000 ± 0.0710, 1.0000 ± 0.0413, P < 0.01). Expression of MMP-2 and MMP-9 in uterus significantly increased at the phase of late-implantation (1.3153 ± 0.3032, 5.0210 ± 4.0307) compared with those of the control (1.0000 ± 0.1771, 1.0000 ± 0.0996, P < 0.01).
Embryo toxicity of CS(2) is more obvious at the phase of late-implantation. Exposure to CS(2) disturbs expression of MMP-2 and MMP-9 in embryo and uterus tissues, which might be one of the important factors contributed to embryo toxicity induced by CS(2).
观察二硫化碳（CS₂）对小鼠胚胎及子宫组织中基质金属蛋白酶（MMP）-2、MMP-9表达的影响，探讨CS₂致胚胎毒性的机制。
在卵泡发育及胚胎着床阶段（分为着床早期和着床晚期），将小鼠腹腔注射CS₂（剂量为631.4mg/kg，体积为0.1ml/10g体重），连续2天。于妊娠第9天获取各项指标，采用明胶酶谱法分析胚胎及子宫组织中MMP-2和MMP-9的表达。
着床晚期暴露后植入胚胎数显著减少（16.000±12.166），与对照组（30.700±5.599，P<0.05）相比。在三个生殖阶段，胚胎中MMP-2和MMP-9的表达明显下降（P<0.01），着床晚期（0.6837±0.0929，0.7309±0.0822）和卵泡发育阶段（0.6222±0.0997，0.7520±0.1068）胚胎中MMP-2和MMP-9的表达水平远低于对照组（1.0000±0.0710，1.0000±0.0413，P<0.01）。着床晚期子宫中MMP-2和MMP-9的表达与对照组（1.0000±0.1771，1.0000±0.0996，P<0.01）相比显著增加（1.3153±0.3032，5.0210±4.0307）。
CS₂的胚胎毒性在着床晚期更明显。暴露于CS₂会干扰胚胎及子宫组织中MMP-2和MMP-9的表达，这可能是CS₂致胚胎毒性的重要因素之一。
