[云南锡矿粉尘诱导的转化上皮细胞中脆性组氨酸三联体（FHIT）蛋白与Ki-67的表达]

Bian Li, He Yong-Wen, Fu Hong-Mei, Ruan Yong-Hua, Gao Qian, Wang Chun-Yan, Jin Ke-Wei

Department of Pathology, the First Affiliated Hospital of Kunming Medical College, Kunming 650031, China.

Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2010 Dec;28(12):895-9.

OBJECTIVE

To study the expression and significance of fragile histidine triad (FHIT) and Ki-67 in transformed epithelial cells induced by Yunnan tin mine dust.

METHODS

Every second generation of immortalized human bronchial epithelial cells (BEAS-2B) and human embryo lung fibroblasts (WI-38) were exposed to 100 µg/ml Yunnan tin mine dust for 72 h, until the ninth generation. The cells were subsequently co-cultured from the 11th generation. Experimental setup: B group, B (W) group, B (W 100) group, B100 group, B100 (W) group, B100 (W100) group. The expressions of FHIT and Ki-67 in epithelial cells were determined by the method of immunocytochemistry at the 16th, 26th and 36th generation. The percentage of Ki-67 positive cells was calculated as proliferation index.

RESULTS

The expression of FHIT was observed in BEAS-2B cells. The expression levels of FHIT among B group, B (W) group and B (W 100) group had not instinctive difference. At the 16th generation, the expression of FHIT in the B100 group was decreased compared with that in the B group and the expression of FHIT between B100 (W) group and B100 (W100) group was lower than that in the B100 group. At the 26th generation, the expression of FHIT was decreased compared with that at the 16th generation in the B100, B100 (W) and B100 (W100) groups. However, At the 36th generation, positive expression were observed again in the B100, B100 (W) and B100 (W100) groups and the expression levels were in incremental order. At the 16th, 26th and 36th generation, the proliferation indexes of B group, B (W) group and B (W 100) group were all < 3%. The proliferation indexes of B100, B100 (W) and B100 (W100) were increased step by step with the generation elongation.

CONCLUSIONS

FHIT could be a target at which Yunnan tin mine dust induces transformation of BEAS-2B cells. The proliferation activation of BEAS-2B cells can be improved by Yunnan tin mine dust.

目的

研究云南锡矿粉尘诱导的转化上皮细胞中脆性组氨酸三联体（FHIT）和Ki-67的表达及意义。

方法

将永生化人支气管上皮细胞（BEAS-2B）和人胚肺成纤维细胞（WI-38）每隔一代用100μg/ml云南锡矿粉尘处理72小时，直至第九代。随后从第11代开始共培养。实验分组：B组、B（W）组、B（W 100）组、B100组、B100（W）组、B100（W100）组。在第16代、26代和36代时，采用免疫细胞化学方法检测上皮细胞中FHIT和Ki-67的表达。计算Ki-67阳性细胞百分比作为增殖指数。

结果

在BEAS-2B细胞中观察到FHIT的表达。B组、B（W）组和B（W 100）组之间FHIT的表达水平无明显差异。在第16代时，B100组中FHIT的表达低于B组，B100（W）组和B100（W100）组中FHIT的表达低于B100组。在第26代时，B100、B100（W）和B100（W100）组中FHIT的表达较第16代降低。然而，在第36代时，B100、B100（W）和B100（W100）组中再次观察到阳性表达，且表达水平呈递增顺序。在第16代、26代和36代时，B组、B（W）组和B（W 100）组的增殖指数均<3%。B100、B100（W）和B100（W100）组的增殖指数随代数延长而逐步增加。