在无 RNase 过程中，使用磁性纳米颗粒进行温度依赖性的质粒 DNA 选择性纯化。
Temperature-dependent selective purification of plasmid DNA using magnetic nanoparticles in an RNase-free process.
机构信息
Faculty of Science, Sichuan Agricultural University, Yaan 625014, People's Republic of China.
出版信息
Anal Biochem. 2011 May 1;412(1):117-9. doi: 10.1016/j.ab.2011.01.012. Epub 2011 Jan 15.
Carboxyl group-functionalized magnetic nanoparticles were used to develop an RNase-free method for plasmid DNA (pDNA) purification directly from RNA-containing crude Escherichia coli lysates. This method takes advantage of differing adsorption behaviors of pDNA and RNA onto magnetic nanoparticle surfaces at different temperatures. Pure pDNA can be isolated between 70 and 80°C without sacrificing DNA quality and quantity, as evidenced by comparison with that obtained using organic solvents or commercial kits. This RNase-free method is rapid, simple, cost-effective, and environmentally friendly, and it can be easily scaled up for the production of pharmacological-grade pDNA.
羧基功能化磁性纳米粒子被用于开发一种无 RNA 酶的方法，可直接从含有 RNA 的粗大肠杆菌裂解物中纯化质粒 DNA（pDNA）。该方法利用了 pDNA 和 RNA 在不同温度下在磁性纳米粒子表面的不同吸附行为。通过与有机溶剂或商业试剂盒获得的结果进行比较，证明在 70 至 80°C 之间可以分离出纯 pDNA，而不会牺牲 DNA 的质量和数量。这种无 RNA 酶的方法快速、简单、具有成本效益且环保，并且可以很容易地扩大规模用于生产药理学级别的 pDNA。
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